Characterization and identification of promoter elements in the mouse COX17 gene

Yoshinori Takahashi, Koichiro Kako, Hidenori Arai, Takahiro Ohishi, Yoshiko Inada, Akio Takehara, Akiyoshi Fukamizu, Eisuke Munekata

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Cox17p, essential for the assembly of functional cytochrome c oxidase (CCO) in Saccharomyces cerevisiae, has been believed to deliver copper ions to the mitochondrion for insertion into the enzyme. We have recently isolated an ∼20 kb genomic fragment of the mouse COX17. Reporter assay experiments have shown that most of the promoter activity was restricted to a 0.85 kb fragment flanking the first exon. Further intensive deletion and detailed mutation analysis suggested that the minimal essential region for transactivation was located at bases -155 to -70. This 5′-flanking region did not possess a TATA box, but contained putative Sp1, NRF-1 and NRF-2 binding sites. COX17 basal promoter activity was abrogated by site-directed mutagenesis of Sp1, NRF-1 and NRF-2 binding sites. Electrophoretic mobility shift assays with AtT-20 and NIH3T3 cell nuclear extract revealed that this region binds both a Sp1-like protein and NRF-1 transcription factors. These results indicated that Sp1, NRF-1 and NRF-2 are involved in basal transcription of the COX17 gene, similar to the transcription mechanism of other CCO-related genes.

Original languageEnglish (US)
Pages (from-to)359-364
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1574
Issue number3
DOIs
StatePublished - Apr 12 2002

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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