Characterization and Proteomic Analysis of Decellularized Adipose Tissue Hydrogels Derived from Lean and Overweight/Obese Human Donors

Omair A. Mohiuddin, Jessica M. Motherwell, Emma Rogers, Melyssa R. Bratton, Qiang Zhang, Guangdi Wang, Bruce Bunnell, Daniel J. Hayes, Jeffrey M. Gimble

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


While decellularized adipose tissue (DAT) has potential as an “off-the-shelf” biomaterial product for regenerative medicine, it remains to be determined if donor-source body mass index (BMI) impacts the functionality of DAT. This study set out to comparatively characterize lean versus overweight/obese-donor derived DAT hydrogel based on proteome and to analyze their respective effects on adipose stromal/stem cell (ASC) viability, and differentiation in vitro. Decellularized adipose tissue from lean (lDAT) and overweight/obese (oDAT) donors is produced and characterized. Variability in the fibril microstructures is found, with dense fibrotic fiber clusters and large pore area uniquely present in the oDAT samples. Proteomic analysis reveals that lDAT contains a greater proportion of enriched extracellular proteins and a smaller proportion of enriched intracellular proteins relative to oDAT. Biocompatibility studies show that ASCs cultured in lDAT and oDAT hydrogels remain viable. The adipogenic and osteogenic differentiation capability of ASCs seeded in lDAT and oDAT hydrogels is confirmed by an upregulation in marker gene expression and phenotypic analysis. In conclusion, this study establishes that DAT hydrogels derived from lean and overweight/obese adipose donors present similar physicochemical profiles with some distinctive features while comparably supporting the viability and adipogenic differentiation of ASCs in vitro.

Original languageEnglish (US)
Article number2000124
JournalAdvanced Biosystems
Issue number10
StatePublished - Oct 1 2020

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering
  • General Biochemistry, Genetics and Molecular Biology


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