Characterization of a cDNA encoding a manganese peroxidase from Phanerochaete chrysosporium: genomic organization of lignin and manganese peroxidase-encoding genes

Ann B. Orth; Margarita Rzhetskaya; Daniel Cullen; Ming Tien

doi:10.1016/0378-1119(94)90251-8

Characterization of a cDNA encoding a manganese peroxidase from Phanerochaete chrysosporium: genomic organization of lignin and manganese peroxidase-encoding genes

Ann B. Orth
, Margarita Rzhetskaya
, Daniel Cullen
, Ming Tien

Plant Institute

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Two heme proteins, manganese peroxidase (MnP) and lignin peroxidase (LiP), play key roles in the fungal depolymerization of lignin. Many cDNA and genomic clones encoding these peroxidases have been published. We report here on the cDNA λMP-2 encoding the MnP isozyme H3 from Phanerochaete chrysosporium strain BKM-F-1767. We also demonstrate that the MnP-encoding gene, λMP-1, encoding isozyme H4, and λMP-2 reside on separate chromosomes from each other and from the LiP-encoding genes. From these results, it is apparent that λMP-2 is not linked to λMP-1 or other genes believed to be involved in lignin depolymerization, such as the LiP and glyoxal oxidase.

Original language	English (US)
Pages (from-to)	161-165
Number of pages	5
Journal	Gene
Volume	148
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(94)90251-8
State	Published - Oct 11 1994

All Science Journal Classification (ASJC) codes

Genetics

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10.1016/0378-1119(94)90251-8

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@article{5db36d7b01c148b592e11cd56199fc14,

title = "Characterization of a cDNA encoding a manganese peroxidase from Phanerochaete chrysosporium: genomic organization of lignin and manganese peroxidase-encoding genes",

abstract = "Two heme proteins, manganese peroxidase (MnP) and lignin peroxidase (LiP), play key roles in the fungal depolymerization of lignin. Many cDNA and genomic clones encoding these peroxidases have been published. We report here on the cDNA λMP-2 encoding the MnP isozyme H3 from Phanerochaete chrysosporium strain BKM-F-1767. We also demonstrate that the MnP-encoding gene, λMP-1, encoding isozyme H4, and λMP-2 reside on separate chromosomes from each other and from the LiP-encoding genes. From these results, it is apparent that λMP-2 is not linked to λMP-1 or other genes believed to be involved in lignin depolymerization, such as the LiP and glyoxal oxidase.",

author = "Orth, \{Ann B.\} and Margarita Rzhetskaya and Daniel Cullen and Ming Tien",

note = "Funding Information: This work was supported in part by U.S. Department of Energy grant DE-FG02-87ER13690 and Public Health Service grant l-P42ES04922-01 from the National Institute of Environmental Health. A.B.O. is a postdoctoral fellow supported by a National Research Service Award (l-F32-ES05503) from the National Institute of Environmental Health.",

year = "1994",

month = oct,

day = "11",

doi = "10.1016/0378-1119(94)90251-8",

language = "English (US)",

volume = "148",

pages = "161--165",

journal = "Gene",

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TY - JOUR

T1 - Characterization of a cDNA encoding a manganese peroxidase from Phanerochaete chrysosporium

T2 - genomic organization of lignin and manganese peroxidase-encoding genes

AU - Orth, Ann B.

AU - Rzhetskaya, Margarita

AU - Cullen, Daniel

AU - Tien, Ming

N1 - Funding Information: This work was supported in part by U.S. Department of Energy grant DE-FG02-87ER13690 and Public Health Service grant l-P42ES04922-01 from the National Institute of Environmental Health. A.B.O. is a postdoctoral fellow supported by a National Research Service Award (l-F32-ES05503) from the National Institute of Environmental Health.

PY - 1994/10/11

Y1 - 1994/10/11

N2 - Two heme proteins, manganese peroxidase (MnP) and lignin peroxidase (LiP), play key roles in the fungal depolymerization of lignin. Many cDNA and genomic clones encoding these peroxidases have been published. We report here on the cDNA λMP-2 encoding the MnP isozyme H3 from Phanerochaete chrysosporium strain BKM-F-1767. We also demonstrate that the MnP-encoding gene, λMP-1, encoding isozyme H4, and λMP-2 reside on separate chromosomes from each other and from the LiP-encoding genes. From these results, it is apparent that λMP-2 is not linked to λMP-1 or other genes believed to be involved in lignin depolymerization, such as the LiP and glyoxal oxidase.

AB - Two heme proteins, manganese peroxidase (MnP) and lignin peroxidase (LiP), play key roles in the fungal depolymerization of lignin. Many cDNA and genomic clones encoding these peroxidases have been published. We report here on the cDNA λMP-2 encoding the MnP isozyme H3 from Phanerochaete chrysosporium strain BKM-F-1767. We also demonstrate that the MnP-encoding gene, λMP-1, encoding isozyme H4, and λMP-2 reside on separate chromosomes from each other and from the LiP-encoding genes. From these results, it is apparent that λMP-2 is not linked to λMP-1 or other genes believed to be involved in lignin depolymerization, such as the LiP and glyoxal oxidase.

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Characterization of a cDNA encoding a manganese peroxidase from Phanerochaete chrysosporium: genomic organization of lignin and manganese peroxidase-encoding genes

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