Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features

Chisato Shimizu; Hiroko Shike; Denise M. Malicki; Eric Breisch; Mark Westerman; John Buchanan; Heather R. Ligman; Ruth B. Phillips; James M. Carlberg; Jack Van Olst; Jane C. Burns

doi:10.1290/1543-706X(2003)039<0029:COAWBM>2.0.CO;2

Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features

Chisato Shimizu, Hiroko Shike, Denise M. Malicki, Eric Breisch, Mark Westerman, John Buchanan, Heather R. Ligman, Ruth B. Phillips, James M. Carlberg, Jack Van Olst, Jane C. Burns

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The genus Morone is an important one for U.S. aquaculture, but there has been no available cell line from this genus. We report here a cell line (the WBE line) derived from white bass embryos that has been grown for more than 80 passages over 21 mo in Dulbecco modified Eagle medium supplemented with fetal bovine serum. The WBE line showed epithelial features with positive immunohistochemical staining for cytokeratin and intercellular junctions when observed by electron microscopy. The doubling time, transformation characteristics, response to cryopreservation, and karyotype were determined. The WBE line was also tested by polymerase chain reaction to verify the species of origin and to screen for mycoplasma infection. This epithelial cell line may prove useful for the study of host-pathogen interactions that occur at the epithelial surface in this commercially important fish species.

Original language	English (US)
Pages (from-to)	29-35
Number of pages	7
Journal	In Vitro Cellular and Developmental Biology - Animal
Volume	39
Issue number	1-2
DOIs	https://doi.org/10.1290/1543-706X(2003)039<0029:COAWBM>2.0.CO;2
State	Published - Jan 2003

All Science Journal Classification (ASJC) codes

Developmental Biology
Cell Biology

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Shimizu, C., Shike, H., Malicki, D. M., Breisch, E., Westerman, M., Buchanan, J., Ligman, H. R., Phillips, R. B., Carlberg, J. M., Van Olst, J., & Burns, J. C. (2003). Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features. In Vitro Cellular and Developmental Biology - Animal, 39(1-2), 29-35. https://doi.org/10.1290/1543-706X(2003)039<0029:COAWBM>2.0.CO;2

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title = "Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features",

abstract = "The genus Morone is an important one for U.S. aquaculture, but there has been no available cell line from this genus. We report here a cell line (the WBE line) derived from white bass embryos that has been grown for more than 80 passages over 21 mo in Dulbecco modified Eagle medium supplemented with fetal bovine serum. The WBE line showed epithelial features with positive immunohistochemical staining for cytokeratin and intercellular junctions when observed by electron microscopy. The doubling time, transformation characteristics, response to cryopreservation, and karyotype were determined. The WBE line was also tested by polymerase chain reaction to verify the species of origin and to screen for mycoplasma infection. This epithelial cell line may prove useful for the study of host-pathogen interactions that occur at the epithelial surface in this commercially important fish species.",

author = "Chisato Shimizu and Hiroko Shike and Malicki, {Denise M.} and Eric Breisch and Mark Westerman and John Buchanan and Ligman, {Heather R.} and Phillips, {Ruth B.} and Carlberg, {James M.} and {Van Olst}, Jack and Burns, {Jane C.}",

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Shimizu, C, Shike, H, Malicki, DM, Breisch, E, Westerman, M, Buchanan, J, Ligman, HR, Phillips, RB, Carlberg, JM, Van Olst, J & Burns, JC 2003, 'Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features', In Vitro Cellular and Developmental Biology - Animal, vol. 39, no. 1-2, pp. 29-35. https://doi.org/10.1290/1543-706X(2003)039<0029:COAWBM>2.0.CO;2

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AU - Shimizu, Chisato

AU - Shike, Hiroko

AU - Malicki, Denise M.

AU - Breisch, Eric

AU - Westerman, Mark

AU - Buchanan, John

AU - Ligman, Heather R.

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AU - Carlberg, James M.

AU - Van Olst, Jack

AU - Burns, Jane C.

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AB - The genus Morone is an important one for U.S. aquaculture, but there has been no available cell line from this genus. We report here a cell line (the WBE line) derived from white bass embryos that has been grown for more than 80 passages over 21 mo in Dulbecco modified Eagle medium supplemented with fetal bovine serum. The WBE line showed epithelial features with positive immunohistochemical staining for cytokeratin and intercellular junctions when observed by electron microscopy. The doubling time, transformation characteristics, response to cryopreservation, and karyotype were determined. The WBE line was also tested by polymerase chain reaction to verify the species of origin and to screen for mycoplasma infection. This epithelial cell line may prove useful for the study of host-pathogen interactions that occur at the epithelial surface in this commercially important fish species.

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Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features

Abstract

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