TY - JOUR
T1 - Characterization of a widely expressed gene (LUC7-LIKE; LUC7L) defining the centromeric boundary of the human α-globin domain
AU - Tufarelli, Cristina
AU - Frischauf, Anna Maria
AU - Hardison, Ross
AU - Flint, Jonathan
AU - Higgs, Douglas R.
N1 - Funding Information:
We thank Helena Ayyub, Jackie Sloane Stanley, and Sue Butler for excellent technical assistance. We also thank Dr. D. Garrick and Dr. D. O’Rourke for helpful discussions and comments on the manuscript. We are grateful to Professor D. J. Weatherall for his continued support and encouragement. Support for this work was provided by the Medical Research Council (C.T. and D.R.H.), NIH Grant DK27635 (R.H.), and the Wellcome Trust (J.F.).
PY - 2001/2/1
Y1 - 2001/2/1
N2 - We have identified the first gene lying on the centromeric side of the α-globin gene cluster on human 16p13.3. The gene, called 16pHQG;16 (HGMW-approved symbol LUC7L), is widely transcribed and lies in the opposite orientation with respect to the α-globin genes. This gene may represent a mammalian heterochromatic gene, encoding a putative RNA-binding protein similar to the yeast Luc7p subunit of the U1 snRNP splicing complex that is normally required for 5′ splice site selection. To examine the role of the 16pHQG;16 gene in delimiting the extent of the α-globin regulatory domain, we mapped its mouse orthologue, which we found to lie on mouse chromosome 17, separated from the mouse α-cluster on chromosome 11. Establishing the full extent of the human 16pHQG;16 gene has allowed us to define the centromeric limit of the region of conserved synteny around the human α-globin cluster to within an 8-kb segment of chromosome 16.
AB - We have identified the first gene lying on the centromeric side of the α-globin gene cluster on human 16p13.3. The gene, called 16pHQG;16 (HGMW-approved symbol LUC7L), is widely transcribed and lies in the opposite orientation with respect to the α-globin genes. This gene may represent a mammalian heterochromatic gene, encoding a putative RNA-binding protein similar to the yeast Luc7p subunit of the U1 snRNP splicing complex that is normally required for 5′ splice site selection. To examine the role of the 16pHQG;16 gene in delimiting the extent of the α-globin regulatory domain, we mapped its mouse orthologue, which we found to lie on mouse chromosome 17, separated from the mouse α-cluster on chromosome 11. Establishing the full extent of the human 16pHQG;16 gene has allowed us to define the centromeric limit of the region of conserved synteny around the human α-globin cluster to within an 8-kb segment of chromosome 16.
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U2 - 10.1006/geno.2000.6394
DO - 10.1006/geno.2000.6394
M3 - Article
C2 - 11170747
AN - SCOPUS:0035251691
SN - 0888-7543
VL - 71
SP - 307
EP - 314
JO - Genomics
JF - Genomics
IS - 3
ER -