TY - JOUR
T1 - Characterization of an HPV type 11 isolate propagated in human foreskin implants in nude mice
AU - Dollard, S. C.
AU - Chow, L. T.
AU - Kreider, J. W.
AU - Broker, T. R.
AU - Lill, N. L.
AU - Howett, M. K.
N1 - Funding Information:
This project was supported by research grants from the USPHS/ NIH CA36200 and CA25305, CA27503, CA4201 1, and Training Grant CA091 24. We thank Dr. Hirohiko Hirochika for advice and Dr. Pierre Chambon for supplying the estrogen receptor expression vector and the vitellogenin enhancer-driven CAT clone. We thank Dr. Mitch 0. Rotenberg for providing the sequence in the E2-C region.
PY - 1989/7
Y1 - 1989/7
N2 - Human papillomavirus type 11-Hershey (HPV-11-H) from an extract of genital warts has been serially passaged by infecting human foreskin chips that are then implanted in athymic mice (1. W. Kreider, M. K. Howett, A. E. Leure-Dupree, R. J. Zaino, and J. A. Weber (1987), J. Virol. 61, 590). Subsequent attempts to propagate HPVs present in other human lesions have not been successful. In an effort to identify the basis for the seemingly unique ability of HPV-11-H to propagate in the xenografts, we carried out extensive physical and functional characterizations of the cloned DNA, including restriction digestions, DNA sequencing of transcriptional control regions, and E2 trans-activation of the upstream regulatory region. We uncovered no significant differences compared to the prototype HPV-11 (K. Dartmann, E. Schwarz, L. Gissmann, and H. zur Hausen (1986) Virology 151,124; H. Hirochika, T. R. Broker, and L. T. Chow (1987) J. Virol. 61, 2599). Moreover, viral enhancer assays indicated that the observed stimulatory effect of pregnancy on condylomata and of estradiol on experimental cysts is likely an indirect one and could not be attributed to up-regulation of the HPV-11 transcriptional enhancer.
AB - Human papillomavirus type 11-Hershey (HPV-11-H) from an extract of genital warts has been serially passaged by infecting human foreskin chips that are then implanted in athymic mice (1. W. Kreider, M. K. Howett, A. E. Leure-Dupree, R. J. Zaino, and J. A. Weber (1987), J. Virol. 61, 590). Subsequent attempts to propagate HPVs present in other human lesions have not been successful. In an effort to identify the basis for the seemingly unique ability of HPV-11-H to propagate in the xenografts, we carried out extensive physical and functional characterizations of the cloned DNA, including restriction digestions, DNA sequencing of transcriptional control regions, and E2 trans-activation of the upstream regulatory region. We uncovered no significant differences compared to the prototype HPV-11 (K. Dartmann, E. Schwarz, L. Gissmann, and H. zur Hausen (1986) Virology 151,124; H. Hirochika, T. R. Broker, and L. T. Chow (1987) J. Virol. 61, 2599). Moreover, viral enhancer assays indicated that the observed stimulatory effect of pregnancy on condylomata and of estradiol on experimental cysts is likely an indirect one and could not be attributed to up-regulation of the HPV-11 transcriptional enhancer.
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U2 - 10.1016/0042-6822(89)90542-4
DO - 10.1016/0042-6822(89)90542-4
M3 - Article
C2 - 2545034
AN - SCOPUS:0024403237
SN - 0042-6822
VL - 171
SP - 294
EP - 297
JO - Virology
JF - Virology
IS - 1
ER -