Characterization of Bacillus subtilis sporulation and bacteriophage infection via FT-IR spectroscopy

Tami H. Mysliwiec; Anthony F. Tierno; David J. Aurentz

doi:10.3233/SPE-2009-0389

Characterization of Bacillus subtilis sporulation and bacteriophage infection via FT-IR spectroscopy

Tami H. Mysliwiec, Anthony F. Tierno, David J. Aurentz

Research output: Contribution to journal › Article › peer-review

Abstract

A comparative analysis on cultures from wild-type and sporulation mutants of Bacillus subtilis infected with pseudotemperate spore-converting bacteriophages was performed to discern differences in the chemical functionality of the cellular population. Variations in the cell surface chemistry of B. subtilis cultures were analyzed using attenuated total reflectance (ATR) Fourier transform infrared (FT-IR) spectroscopy of dried sample films on optical plates. Comparison of wild-type and phage infected cultures revealed an increase in the amount of aliphatic ester in the infected populations. Spectral analysis of cultures from bacteriophage infected sporulation mutants, spo0K and spo0J revealed varying levels of ester linkages.

Original language	English (US)
Pages (from-to)	165-174
Number of pages	10
Journal	Spectroscopy
Volume	23
Issue number	3-4
DOIs	https://doi.org/10.3233/SPE-2009-0389
State	Published - 2009

All Science Journal Classification (ASJC) codes

Spectroscopy

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10.3233/SPE-2009-0389

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title = "Characterization of Bacillus subtilis sporulation and bacteriophage infection via FT-IR spectroscopy",

abstract = "A comparative analysis on cultures from wild-type and sporulation mutants of Bacillus subtilis infected with pseudotemperate spore-converting bacteriophages was performed to discern differences in the chemical functionality of the cellular population. Variations in the cell surface chemistry of B. subtilis cultures were analyzed using attenuated total reflectance (ATR) Fourier transform infrared (FT-IR) spectroscopy of dried sample films on optical plates. Comparison of wild-type and phage infected cultures revealed an increase in the amount of aliphatic ester in the infected populations. Spectral analysis of cultures from bacteriophage infected sporulation mutants, spo0K and spo0J revealed varying levels of ester linkages.",

author = "Mysliwiec, {Tami H.} and Tierno, {Anthony F.} and Aurentz, {David J.}",

year = "2009",

doi = "10.3233/SPE-2009-0389",

language = "English (US)",

volume = "23",

pages = "165--174",

journal = "Spectroscopy",

issn = "0712-4813",

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AU - Tierno, Anthony F.

AU - Aurentz, David J.

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N2 - A comparative analysis on cultures from wild-type and sporulation mutants of Bacillus subtilis infected with pseudotemperate spore-converting bacteriophages was performed to discern differences in the chemical functionality of the cellular population. Variations in the cell surface chemistry of B. subtilis cultures were analyzed using attenuated total reflectance (ATR) Fourier transform infrared (FT-IR) spectroscopy of dried sample films on optical plates. Comparison of wild-type and phage infected cultures revealed an increase in the amount of aliphatic ester in the infected populations. Spectral analysis of cultures from bacteriophage infected sporulation mutants, spo0K and spo0J revealed varying levels of ester linkages.

AB - A comparative analysis on cultures from wild-type and sporulation mutants of Bacillus subtilis infected with pseudotemperate spore-converting bacteriophages was performed to discern differences in the chemical functionality of the cellular population. Variations in the cell surface chemistry of B. subtilis cultures were analyzed using attenuated total reflectance (ATR) Fourier transform infrared (FT-IR) spectroscopy of dried sample films on optical plates. Comparison of wild-type and phage infected cultures revealed an increase in the amount of aliphatic ester in the infected populations. Spectral analysis of cultures from bacteriophage infected sporulation mutants, spo0K and spo0J revealed varying levels of ester linkages.

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DO - 10.3233/SPE-2009-0389

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JO - Spectroscopy

JF - Spectroscopy

IS - 3-4

ER -

Characterization of Bacillus subtilis sporulation and bacteriophage infection via FT-IR spectroscopy

Abstract

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