TY - JOUR
T1 - Characterization of bacterial communities in solarized soil amended with lignocellulosic organic matter
AU - Simmons, Christopher W.
AU - Claypool, Joshua T.
AU - Marshall, Megan N.
AU - Jabusch, Lauren K.
AU - Reddy, Amitha P.
AU - Simmons, Blake A.
AU - Singer, Steven W.
AU - Stapleton, James J.
AU - VanderGheynst, Jean S.
N1 - Funding Information:
The authors thank Dr. Ruth Dahlquist and Fresno Pacific University students Stacy Betts, Katie Hernandez, and Naomi Bogonko, for assistance with the solarization field experiments and Dr. Michael Raviv at Newe Ya’ar Research Center at the Agricultural Research Organization of Israel, for discussions related to field experiments. This work was funded by the United States-Israel Binational Agricultural Research and Development Fund #US-4266-09 R and the UC Laboratory Fees Research Program #12-LR-237496, and was performed as part of the DOE Joint BioEnergy Institute ( http://www.jbei.org ) supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U.S. Department of Energy. The financial sponsors of this work had no role in study design; in the collection, analysis and interpretation of data; in the writing of the article; and in the decision to submit the article for publication. 16S rRNA gene sequencing was conducted by the Joint Genome Institute, which is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
PY - 2014/1
Y1 - 2014/1
N2 - Solarization can provide thermal inactivation of weed seeds and phytopathogens through passive solar heating of moist soil covered with clear plastic tarp. Microbial respiration in soils, especially those with increasing levels of organic matter, can augment solarization to produce soil temperatures higher than those achieved by solar heating alone. Currently, it is unclear how solarization affects microbial community structure in soils amended with organic matter to promote microbial activity. In this study, a field trial was conducted in the San Joaquin Valley of California to solarize an irrigated, agricultural field soil which was either amended with mature green waste compost destabilized with wheat bran, or not amended. Following 22 days of treatment during July-August 2011, soil from three depth increments (0-5.8, 5.8-11.6, and 11.6-17.4. cm) was subjected to 16S ribosomal RNA gene sequencing to characterize microbial communities. The sequencing data obtained revealed similar microbial species richness and evenness in both solarized amended and non-amended soil. However, the taxonomic composition of communities differed by treatment. Furthermore, community structure within each treatment changed with soil depth, indicating potential enrichment of thermophilic bacteria in layers that experienced greatest heating, as well as changes related to alterations in the soil atmosphere. Certain bacteria detected in solarized, compost-amended soil may be relevant to agriculture and plant biomass deconstruction processes.
AB - Solarization can provide thermal inactivation of weed seeds and phytopathogens through passive solar heating of moist soil covered with clear plastic tarp. Microbial respiration in soils, especially those with increasing levels of organic matter, can augment solarization to produce soil temperatures higher than those achieved by solar heating alone. Currently, it is unclear how solarization affects microbial community structure in soils amended with organic matter to promote microbial activity. In this study, a field trial was conducted in the San Joaquin Valley of California to solarize an irrigated, agricultural field soil which was either amended with mature green waste compost destabilized with wheat bran, or not amended. Following 22 days of treatment during July-August 2011, soil from three depth increments (0-5.8, 5.8-11.6, and 11.6-17.4. cm) was subjected to 16S ribosomal RNA gene sequencing to characterize microbial communities. The sequencing data obtained revealed similar microbial species richness and evenness in both solarized amended and non-amended soil. However, the taxonomic composition of communities differed by treatment. Furthermore, community structure within each treatment changed with soil depth, indicating potential enrichment of thermophilic bacteria in layers that experienced greatest heating, as well as changes related to alterations in the soil atmosphere. Certain bacteria detected in solarized, compost-amended soil may be relevant to agriculture and plant biomass deconstruction processes.
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U2 - 10.1016/j.apsoil.2013.08.014
DO - 10.1016/j.apsoil.2013.08.014
M3 - Article
AN - SCOPUS:84884374205
SN - 0929-1393
VL - 73
SP - 97
EP - 104
JO - Applied Soil Ecology
JF - Applied Soil Ecology
ER -