TY - JOUR
T1 - Characterization of feruloyl esterases in maize pollen
AU - de O Buanafina, Marcia M.
AU - Fernanda Buanafina, M.
AU - Laremore, Tatiana
AU - Shearer, Erica A.
AU - Fescemyer, Howard W.
N1 - Funding Information:
This research was supported by the U.S. Department of Agriculture (USDA), grant number 2009-35318-0513, Department of Energy (DOE) Plant Feedstock Genomics Research Program, grant number DE-FG02-08ER64701 to MB. Small part of funding support for this work was by the Elberly College of Science, PSU. We thank Dr. T. Ishii (Japan) for supplying the feruloylated oligosaccharides (FAXX) sb, Dr Scott Borneman, Genencor, USA, for supplying feruloylated polysaccharides (FAXX) w , FAXXX, FXG, p CAXX, FAA and FAAA; Dr. B. Minkenberg (The Pennsylvania State University) for supplying rice plants for pollen collection, Dr. P. Adler and Mr. M. Mayers (USDA-ARS) for supplying big bluestem and Indian grasses for pollen collection, Mr. A. Omeis for supplying pollen from ornamental plants, Dr. M.C. Saha (Samuel Roberts Noble Foundation) for supplying the tall fescue genotype for pollen collection, Mr. P. Mush and Dr. D. Thorogood (IBERS/UK) for supplying Lolium plants for pollen collection, and Dr. P. Morris for discussions on the manuscript.
Funding Information:
This research was supported by the U.S. Department of Agriculture (USDA), grant number 2009-35318-0513, Department of Energy (DOE) Plant Feedstock Genomics Research Program, grant number DE-FG02-08ER64701 to MB. Small part of funding support for this work was by the Elberly College of Science, PSU. We thank Dr. T. Ishii (Japan) for supplying the feruloylated oligosaccharides (FAXX)sb, Dr Scott Borneman, Genencor, USA, for supplying feruloylated polysaccharides (FAXX)w , FAXXX, FXG, p CAXX, FAA and FAAA; Dr. B. Minkenberg (The Pennsylvania State University) for supplying rice plants for pollen collection, Dr. P. Adler and Mr. M. Mayers (USDA-ARS) for supplying big bluestem and Indian grasses for pollen collection, Mr. A. Omeis for supplying pollen from ornamental plants, Dr. M.C. Saha (Samuel Roberts Noble Foundation) for supplying the tall fescue genotype for pollen collection, Mr. P. Mush and Dr. D. Thorogood (IBERS/UK) for supplying Lolium plants for pollen collection, and Dr. P. Morris for discussions on the manuscript.
Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Main conclusion: Ferulic acid esterases have been identified and partially purified from maize pollen. Results suggest that maize pollen FAEs may play an important role in pollen fertilization. Abstract: A critical step in maize (Zea mays) seed production involves fertilization of the ovule by pollen, a process that relies on ability of the pollen tube to grow through the highly structured and feruloylated arabinoxylan/cellulose-rich tissue of the silk and stigma. It is known that different cell wall hydrolases are present on the surface of pollen. An important hydrolase reported to date is an endo-xylanase (ZmXYN1). We report presence and characterization of another hydrolase, ferulic acid esterase (FAE), in maize pollen. Using a combination of biochemical approaches, these FAEs were partially purified and characterized with respect to their biochemical properties and putative sequences. Maize pollen FAEs were shown to be expressed early during pollen development, to release significant amounts of both monomeric and dimeric ferulates esterified from maize silks and other grass cell walls, and to synergize with an externally applied fungal endo-1,4-β-xylanase on the release of cell wall ferulates and diferulates. Preliminary analysis of maize silk cell walls following pollination, showed a significant reduction of esterified ferulates up to 96 h following pollination, compared to unpollinated silks. These results suggest that maize pollen FAEs may play an important biological role in pollen fertilization and possibly in seed production.
AB - Main conclusion: Ferulic acid esterases have been identified and partially purified from maize pollen. Results suggest that maize pollen FAEs may play an important role in pollen fertilization. Abstract: A critical step in maize (Zea mays) seed production involves fertilization of the ovule by pollen, a process that relies on ability of the pollen tube to grow through the highly structured and feruloylated arabinoxylan/cellulose-rich tissue of the silk and stigma. It is known that different cell wall hydrolases are present on the surface of pollen. An important hydrolase reported to date is an endo-xylanase (ZmXYN1). We report presence and characterization of another hydrolase, ferulic acid esterase (FAE), in maize pollen. Using a combination of biochemical approaches, these FAEs were partially purified and characterized with respect to their biochemical properties and putative sequences. Maize pollen FAEs were shown to be expressed early during pollen development, to release significant amounts of both monomeric and dimeric ferulates esterified from maize silks and other grass cell walls, and to synergize with an externally applied fungal endo-1,4-β-xylanase on the release of cell wall ferulates and diferulates. Preliminary analysis of maize silk cell walls following pollination, showed a significant reduction of esterified ferulates up to 96 h following pollination, compared to unpollinated silks. These results suggest that maize pollen FAEs may play an important biological role in pollen fertilization and possibly in seed production.
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U2 - 10.1007/s00425-019-03288-y
DO - 10.1007/s00425-019-03288-y
M3 - Article
C2 - 31576447
AN - SCOPUS:85074176268
SN - 0032-0935
VL - 250
SP - 2063
EP - 2082
JO - Planta
JF - Planta
IS - 6
ER -