Characterization of mtDNA SNP typing using quantitative real-time PCR with special emphasis on heteroplasmy detection and mixture ratio assessment

H. Niederstätter; M. D. Coble; T. J. Parsons; W. Parson

doi:10.1016/j.ics.2005.09.021

Characterization of mtDNA SNP typing using quantitative real-time PCR with special emphasis on heteroplasmy detection and mixture ratio assessment

H. Niederstätter
, M. D. Coble
, T. J. Parsons
, W. Parson

Forensic Science Program

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Using a plasmid DNA model and total genomic DNA (gDNA) reference samples, we examined the performance and forensic utility of a quantitative allele-discriminatory real-time PCR assay relying on TaqMan probes targeting the mitochondrial single nucleotide polymorphism (mtSNP) 16519T/C. For allele-calling and allele proportion assessment we used absolute quantification of both alleles as well as relative quantification based on endpoint fluorescence.

Original language	English (US)
Pages (from-to)	1-3
Number of pages	3
Journal	International Congress Series
Volume	1288
DOIs	https://doi.org/10.1016/j.ics.2005.09.021
State	Published - Apr 2006

All Science Journal Classification (ASJC) codes

General Medicine

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10.1016/j.ics.2005.09.021

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title = "Characterization of mtDNA SNP typing using quantitative real-time PCR with special emphasis on heteroplasmy detection and mixture ratio assessment",

abstract = "Using a plasmid DNA model and total genomic DNA (gDNA) reference samples, we examined the performance and forensic utility of a quantitative allele-discriminatory real-time PCR assay relying on TaqMan probes targeting the mitochondrial single nucleotide polymorphism (mtSNP) 16519T/C. For allele-calling and allele proportion assessment we used absolute quantification of both alleles as well as relative quantification based on endpoint fluorescence.",

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note = "Funding Information: Part of this work was supported by the National Institute of Justice grant 2000-1J-CX-K010 to T.J.P. The opinions and assertions contained herein are solely those of the authors and are not to be construed as official or as views of the U.S. Department of Defense, the U.S. Department of the Army, or the U.S. Department of Justice.",

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AU - Parsons, T. J.

AU - Parson, W.

N1 - Funding Information: Part of this work was supported by the National Institute of Justice grant 2000-1J-CX-K010 to T.J.P. The opinions and assertions contained herein are solely those of the authors and are not to be construed as official or as views of the U.S. Department of Defense, the U.S. Department of the Army, or the U.S. Department of Justice.

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N2 - Using a plasmid DNA model and total genomic DNA (gDNA) reference samples, we examined the performance and forensic utility of a quantitative allele-discriminatory real-time PCR assay relying on TaqMan probes targeting the mitochondrial single nucleotide polymorphism (mtSNP) 16519T/C. For allele-calling and allele proportion assessment we used absolute quantification of both alleles as well as relative quantification based on endpoint fluorescence.

AB - Using a plasmid DNA model and total genomic DNA (gDNA) reference samples, we examined the performance and forensic utility of a quantitative allele-discriminatory real-time PCR assay relying on TaqMan probes targeting the mitochondrial single nucleotide polymorphism (mtSNP) 16519T/C. For allele-calling and allele proportion assessment we used absolute quantification of both alleles as well as relative quantification based on endpoint fluorescence.

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JO - International Congress Series

JF - International Congress Series

ER -

Characterization of mtDNA SNP typing using quantitative real-time PCR with special emphasis on heteroplasmy detection and mixture ratio assessment

Abstract

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