Characterization of plasmids encoding type II restriction - Modification systems, isoschizomers of SsoII

M. M. Den'mukhametov; M. V. Zakharova; A. N. Kravets; A. V. Pertsev; E. V. Sineva; A. V. Repik; I. V. Beletskaya; E. S. Gromova; A. S. Solonin

Characterization of plasmids encoding type II restriction - Modification systems, isoschizomers of SsoII

M. M. Den'mukhametov, M. V. Zakharova, A. N. Kravets, A. V. Pertsev, E. V. Sineva, A. V. Repik, I. V. Beletskaya, E. S. Gromova, A. S. Solonin

Biochemistry & Molecular Biology

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4 Scopus citations

Abstract

Five strains of Enterobacteriaceae family have been revealed that have restriction-modification systems of type II, isoschizomers of SsoII. The genes coding for the restriction endonucleases and DNA methyltransferases are located on plasmids of two types. These plasmids differ in size, capacity for mobilization by conjugative plasmids, and structure of locus cer. Recombinant two-plasmid strains were obtained containing plasmids with genes for Ecl18kI and EcoRII restriction endonucleases in the presence of DNA methyltransferase Ecl18kI. The stability of the two-plasmid system with the ecoRIIR and ecl18kIM genes implies similar mechanisms of concerted regulation of gene expression in the restriction-modification systems Ecl18kI and EcoRII. Genes for Ecl18kI and SsoII restriction-modification systems have essentially the same sequence.

Original language	English (US)
Pages (from-to)	708-714
Number of pages	7
Journal	Molecular Biology
Volume	31
Issue number	5
State	Published - Sep 1997

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology

Cite this

@article{94408b33ea8b4f09919ff59c3875c05b,

title = "Characterization of plasmids encoding type II restriction - Modification systems, isoschizomers of SsoII",

abstract = "Five strains of Enterobacteriaceae family have been revealed that have restriction-modification systems of type II, isoschizomers of SsoII. The genes coding for the restriction endonucleases and DNA methyltransferases are located on plasmids of two types. These plasmids differ in size, capacity for mobilization by conjugative plasmids, and structure of locus cer. Recombinant two-plasmid strains were obtained containing plasmids with genes for Ecl18kI and EcoRII restriction endonucleases in the presence of DNA methyltransferase Ecl18kI. The stability of the two-plasmid system with the ecoRIIR and ecl18kIM genes implies similar mechanisms of concerted regulation of gene expression in the restriction-modification systems Ecl18kI and EcoRII. Genes for Ecl18kI and SsoII restriction-modification systems have essentially the same sequence.",

author = "Den'mukhametov, {M. M.} and Zakharova, {M. V.} and Kravets, {A. N.} and Pertsev, {A. V.} and Sineva, {E. V.} and Repik, {A. V.} and Beletskaya, {I. V.} and Gromova, {E. S.} and Solonin, {A. S.}",

year = "1997",

month = sep,

language = "English (US)",

volume = "31",

pages = "708--714",

journal = "Molecular Biology",

issn = "0026-8933",

publisher = "Pleiades Publishing",

number = "5",

}

TY - JOUR

T1 - Characterization of plasmids encoding type II restriction - Modification systems, isoschizomers of SsoII

AU - Den'mukhametov, M. M.

AU - Zakharova, M. V.

AU - Kravets, A. N.

AU - Pertsev, A. V.

AU - Sineva, E. V.

AU - Repik, A. V.

AU - Beletskaya, I. V.

AU - Gromova, E. S.

AU - Solonin, A. S.

PY - 1997/9

Y1 - 1997/9

N2 - Five strains of Enterobacteriaceae family have been revealed that have restriction-modification systems of type II, isoschizomers of SsoII. The genes coding for the restriction endonucleases and DNA methyltransferases are located on plasmids of two types. These plasmids differ in size, capacity for mobilization by conjugative plasmids, and structure of locus cer. Recombinant two-plasmid strains were obtained containing plasmids with genes for Ecl18kI and EcoRII restriction endonucleases in the presence of DNA methyltransferase Ecl18kI. The stability of the two-plasmid system with the ecoRIIR and ecl18kIM genes implies similar mechanisms of concerted regulation of gene expression in the restriction-modification systems Ecl18kI and EcoRII. Genes for Ecl18kI and SsoII restriction-modification systems have essentially the same sequence.

AB - Five strains of Enterobacteriaceae family have been revealed that have restriction-modification systems of type II, isoschizomers of SsoII. The genes coding for the restriction endonucleases and DNA methyltransferases are located on plasmids of two types. These plasmids differ in size, capacity for mobilization by conjugative plasmids, and structure of locus cer. Recombinant two-plasmid strains were obtained containing plasmids with genes for Ecl18kI and EcoRII restriction endonucleases in the presence of DNA methyltransferase Ecl18kI. The stability of the two-plasmid system with the ecoRIIR and ecl18kIM genes implies similar mechanisms of concerted regulation of gene expression in the restriction-modification systems Ecl18kI and EcoRII. Genes for Ecl18kI and SsoII restriction-modification systems have essentially the same sequence.

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UR - http://www.scopus.com/inward/citedby.url?scp=0031312462&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0031312462

SN - 0026-8933

VL - 31

SP - 708

EP - 714

JO - Molecular Biology

JF - Molecular Biology

IS - 5

ER -

Characterization of plasmids encoding type II restriction - Modification systems, isoschizomers of SsoII

Abstract

All Science Journal Classification (ASJC) codes

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