Characterization of protein kinases in mitotic and meiotic cell extracts

Margaret S. Halleck, Katherine Lumley-Sapanski, Jon A. Reed, Anand P. Iyer, Andrea M. Mastro, Robert A. Schlegel

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


A number of protein kinases have been separated and identified in extracts from mitotic and interphase culture cells and from mature and immature amphibian oocytes using nondenaturing polyacrylamide gel electrophoresis followed by in situ phosphorylation assays. Certain of these protein kinase activities appear to correlate with the biological activity of extracts, assayed by their ability to induce meiotic maturation following injection into Xenopus oocytes. These results are consistent with the notion that protein phosphorylation/dephosphorylation may be integral to the mechanisms of both nuclear membrane breakdown and chromosome condensation, events common and distinctive to mitosis and meiosis.

Original languageEnglish (US)
Pages (from-to)193-198
Number of pages6
JournalFEBS Letters
Issue number2
StatePublished - Feb 27 1984

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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