Characterization of the avian Ich-1 cDNA and expression of Ich-1(L) mRNA in the hen ovary

A. L. Johnson; J. T. Bridgham; L. Bergeron; J. Yuan

doi:10.1016/S0378-1119(97)00068-1

Characterization of the avian Ich-1 cDNA and expression of Ich-1(L) mRNA in the hen ovary

A. L. Johnson
, J. T. Bridgham
, L. Bergeron
, J. Yuan

Animal Science

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We have sequenced the chicken interleukin-1β-converting enzyme (ICE) and ced-3 homolog (Ich-1) cDNA, and evaluated Ich-1 mRNA expression in the hen ovary during follicle development. While two alternatively spliced forms of Ich-1, Ich-1(L) and Ich-1(S), were amplified by PCR from an embryonic chicken cDNA library, only the Ich-1(L) form was found to be expressed in adult ovarian granulosa and theca tissues. The deduced amino acid (aa) sequence of ICH-1(L) is 70.8% identical to human ICH-1(L) and contains the conserved QACRG peptide active catalytic sequence characteristic of many ICE-related family of cysteine proteases.

Original language	English (US)
Pages (from-to)	227-233
Number of pages	7
Journal	Gene
Volume	192
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1119(97)00068-1
State	Published - Jun 19 1997

All Science Journal Classification (ASJC) codes

Genetics

Access to Document

10.1016/S0378-1119(97)00068-1

Cite this

@article{7c787da2ed574aa2a89ff0b5cb6a321f,

title = "Characterization of the avian Ich-1 cDNA and expression of Ich-1(L) mRNA in the hen ovary",

abstract = "We have sequenced the chicken interleukin-1β-converting enzyme (ICE) and ced-3 homolog (Ich-1) cDNA, and evaluated Ich-1 mRNA expression in the hen ovary during follicle development. While two alternatively spliced forms of Ich-1, Ich-1(L) and Ich-1(S), were amplified by PCR from an embryonic chicken cDNA library, only the Ich-1(L) form was found to be expressed in adult ovarian granulosa and theca tissues. The deduced amino acid (aa) sequence of ICH-1(L) is 70.8\% identical to human ICH-1(L) and contains the conserved QACRG peptide active catalytic sequence characteristic of many ICE-related family of cysteine proteases.",

author = "Johnson, \{A. L.\} and Bridgham, \{J. T.\} and L. Bergeron and J. Yuan",

note = "Funding Information: We thank Dr. J.L. Tilly (Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston) and Dr. J.P. Witty for valuable discussions and comments during the course of these studies. Supported by USDA grant 95-37203-1998 (to A.L.J.) and from the National Institute of Aging and Bristol Myer-Squibb (to J.Y.).",

year = "1997",

month = jun,

day = "19",

doi = "10.1016/S0378-1119(97)00068-1",

language = "English (US)",

volume = "192",

pages = "227--233",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Characterization of the avian Ich-1 cDNA and expression of Ich-1(L) mRNA in the hen ovary

AU - Johnson, A. L.

AU - Bridgham, J. T.

AU - Bergeron, L.

AU - Yuan, J.

N1 - Funding Information: We thank Dr. J.L. Tilly (Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston) and Dr. J.P. Witty for valuable discussions and comments during the course of these studies. Supported by USDA grant 95-37203-1998 (to A.L.J.) and from the National Institute of Aging and Bristol Myer-Squibb (to J.Y.).

PY - 1997/6/19

Y1 - 1997/6/19

N2 - We have sequenced the chicken interleukin-1β-converting enzyme (ICE) and ced-3 homolog (Ich-1) cDNA, and evaluated Ich-1 mRNA expression in the hen ovary during follicle development. While two alternatively spliced forms of Ich-1, Ich-1(L) and Ich-1(S), were amplified by PCR from an embryonic chicken cDNA library, only the Ich-1(L) form was found to be expressed in adult ovarian granulosa and theca tissues. The deduced amino acid (aa) sequence of ICH-1(L) is 70.8% identical to human ICH-1(L) and contains the conserved QACRG peptide active catalytic sequence characteristic of many ICE-related family of cysteine proteases.

AB - We have sequenced the chicken interleukin-1β-converting enzyme (ICE) and ced-3 homolog (Ich-1) cDNA, and evaluated Ich-1 mRNA expression in the hen ovary during follicle development. While two alternatively spliced forms of Ich-1, Ich-1(L) and Ich-1(S), were amplified by PCR from an embryonic chicken cDNA library, only the Ich-1(L) form was found to be expressed in adult ovarian granulosa and theca tissues. The deduced amino acid (aa) sequence of ICH-1(L) is 70.8% identical to human ICH-1(L) and contains the conserved QACRG peptide active catalytic sequence characteristic of many ICE-related family of cysteine proteases.

UR - https://www.scopus.com/pages/publications/0030993301

UR - https://www.scopus.com/pages/publications/0030993301#tab=citedBy

U2 - 10.1016/S0378-1119(97)00068-1

DO - 10.1016/S0378-1119(97)00068-1

M3 - Article

C2 - 9224894

AN - SCOPUS:0030993301

SN - 0378-1119

VL - 192

SP - 227

EP - 233

JO - Gene

JF - Gene

IS - 2

ER -

Characterization of the avian Ich-1 cDNA and expression of Ich-1(L) mRNA in the hen ovary

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this