TY - JOUR
T1 - Characterization of the Lipid Structure and Fluidity of Lipid Membranes on Epitaxial Graphene and Their Correlation to Graphene Features
AU - Farell, Megan
AU - Wetherington, Maxwell
AU - Shankla, Manish
AU - Chae, Inseok
AU - Subramanian, Shruti
AU - Kim, Seong H.
AU - Aksimentiev, Aleksei
AU - Robinson, Joshua
AU - Kumar, Manish
N1 - Funding Information:
This material is based upon work supported by the National Science Foundation Graduate Research Fellowship Program under grant no. DGE1255832. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. S.S. and J.A.R. acknowledge the funding from NSF CAREER (award: 1453924) which supported this research. Funding was also provided by Penn State University as a part of a Materials Research Institute Huck Institute of Life Sciences seed grant. The computational studies were supported by the grants from the National Institutes of Health R01-HG007406 and P41-GM104601. M.S. and A.A. acknowledge supercomputer time provided through the XSEDE Allocation Grant MCA05S028 and the Blue Waters petascale supercomputer system at the University of Illinois at Urbana−Champaign. We thank Dr. Paul Cremer and his group for their assistance with FRAP experiments on a traditional setup and their insight on formation of supported lipid structures.
Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/4/2
Y1 - 2019/4/2
N2 - Graphene has been recognized as an enhanced platform for biosensors because of its high electron mobility. To integrate active membrane proteins into graphene-based materials for such applications, graphene's surface must be functionalized with lipids to mimic the biological environment of these proteins. Several studies have examined supported lipids on various types of graphene and obtained conflicting results for the lipid structure. Here, we present a correlative characterization technique based on fluorescence measurements in a Raman spectroscopy setup to study the lipid structure and dynamics on epitaxial graphene. Compared to other graphene variations, epitaxial graphene is grown on a substrate more conducive to production of electronics and offers unique topographic features. On the basis of experimental and computational results, we propose that a lipid sesquilayer (1.5 bilayer) forms on epitaxial graphene and demonstrate that the distinct surface features of epitaxial graphene affect the structure and diffusion of supported lipids.
AB - Graphene has been recognized as an enhanced platform for biosensors because of its high electron mobility. To integrate active membrane proteins into graphene-based materials for such applications, graphene's surface must be functionalized with lipids to mimic the biological environment of these proteins. Several studies have examined supported lipids on various types of graphene and obtained conflicting results for the lipid structure. Here, we present a correlative characterization technique based on fluorescence measurements in a Raman spectroscopy setup to study the lipid structure and dynamics on epitaxial graphene. Compared to other graphene variations, epitaxial graphene is grown on a substrate more conducive to production of electronics and offers unique topographic features. On the basis of experimental and computational results, we propose that a lipid sesquilayer (1.5 bilayer) forms on epitaxial graphene and demonstrate that the distinct surface features of epitaxial graphene affect the structure and diffusion of supported lipids.
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U2 - 10.1021/acs.langmuir.9b00164
DO - 10.1021/acs.langmuir.9b00164
M3 - Article
C2 - 30844287
AN - SCOPUS:85063472725
SN - 0743-7463
VL - 35
SP - 4726
EP - 4735
JO - Langmuir
JF - Langmuir
IS - 13
ER -