Characterization of viral siRNA populations in honey bee colony collapse disorder

Nor Chejanovsky; Ron Ophir; Michal Sharabi Schwager; Yossi Slabezki; Smadar Grossman; Diana Cox-Foster

doi:10.1016/j.virol.2014.02.012

Characterization of viral siRNA populations in honey bee colony collapse disorder

Nor Chejanovsky, Ron Ophir, Michal Sharabi Schwager, Yossi Slabezki, Smadar Grossman, Diana Cox-Foster

Entomology

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

Colony Collapse Disorder (CCD), a special case of collapse of honey bee colonies, has resulted in significant losses for beekeepers. CCD-colonies show abundance of pathogens which suggests that they have a weakened immune system. Since honey bee viruses are major players in colony collapse and given the important role of viral RNA interference (RNAi) in combating viral infections we investigated if CCD-colonies elicit an RNAi response. Deep-sequencing analysis of samples from CCD-colonies from US and Israel revealed abundant small interfering RNAs (siRNA) of 21-22 nucleotides perfectly matching the Israeli acute paralysis virus (IAPV), Kashmir virus and Deformed wing virus genomes. Israeli colonies showed high titers of IAPV and a conserved RNAi-pattern of matching the viral genome. That was also observed in sample analysis from colonies experimentally infected with IAPV. Our results suggest that CCD-colonies set out a siRNA response that is specific against predominant viruses associated with colony losses.

Original language	English (US)
Pages (from-to)	176-183
Number of pages	8
Journal	Virology
Volume	454-455
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2014.02.012
State	Published - Apr 2014

All Science Journal Classification (ASJC) codes

Virology

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10.1016/j.virol.2014.02.012

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title = "Characterization of viral siRNA populations in honey bee colony collapse disorder",

abstract = "Colony Collapse Disorder (CCD), a special case of collapse of honey bee colonies, has resulted in significant losses for beekeepers. CCD-colonies show abundance of pathogens which suggests that they have a weakened immune system. Since honey bee viruses are major players in colony collapse and given the important role of viral RNA interference (RNAi) in combating viral infections we investigated if CCD-colonies elicit an RNAi response. Deep-sequencing analysis of samples from CCD-colonies from US and Israel revealed abundant small interfering RNAs (siRNA) of 21-22 nucleotides perfectly matching the Israeli acute paralysis virus (IAPV), Kashmir virus and Deformed wing virus genomes. Israeli colonies showed high titers of IAPV and a conserved RNAi-pattern of matching the viral genome. That was also observed in sample analysis from colonies experimentally infected with IAPV. Our results suggest that CCD-colonies set out a siRNA response that is specific against predominant viruses associated with colony losses.",

author = "Nor Chejanovsky and Ron Ophir and Schwager, {Michal Sharabi} and Yossi Slabezki and Smadar Grossman and Diana Cox-Foster",

note = "Funding Information: This work was supported by the Binational Agricultural Research and Development Fund (BARD) under Grant no. IS-4920-10 .",

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doi = "10.1016/j.virol.2014.02.012",

language = "English (US)",

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T1 - Characterization of viral siRNA populations in honey bee colony collapse disorder

AU - Chejanovsky, Nor

AU - Ophir, Ron

AU - Schwager, Michal Sharabi

AU - Slabezki, Yossi

AU - Grossman, Smadar

AU - Cox-Foster, Diana

N1 - Funding Information: This work was supported by the Binational Agricultural Research and Development Fund (BARD) under Grant no. IS-4920-10 .

PY - 2014/4

Y1 - 2014/4

N2 - Colony Collapse Disorder (CCD), a special case of collapse of honey bee colonies, has resulted in significant losses for beekeepers. CCD-colonies show abundance of pathogens which suggests that they have a weakened immune system. Since honey bee viruses are major players in colony collapse and given the important role of viral RNA interference (RNAi) in combating viral infections we investigated if CCD-colonies elicit an RNAi response. Deep-sequencing analysis of samples from CCD-colonies from US and Israel revealed abundant small interfering RNAs (siRNA) of 21-22 nucleotides perfectly matching the Israeli acute paralysis virus (IAPV), Kashmir virus and Deformed wing virus genomes. Israeli colonies showed high titers of IAPV and a conserved RNAi-pattern of matching the viral genome. That was also observed in sample analysis from colonies experimentally infected with IAPV. Our results suggest that CCD-colonies set out a siRNA response that is specific against predominant viruses associated with colony losses.

AB - Colony Collapse Disorder (CCD), a special case of collapse of honey bee colonies, has resulted in significant losses for beekeepers. CCD-colonies show abundance of pathogens which suggests that they have a weakened immune system. Since honey bee viruses are major players in colony collapse and given the important role of viral RNA interference (RNAi) in combating viral infections we investigated if CCD-colonies elicit an RNAi response. Deep-sequencing analysis of samples from CCD-colonies from US and Israel revealed abundant small interfering RNAs (siRNA) of 21-22 nucleotides perfectly matching the Israeli acute paralysis virus (IAPV), Kashmir virus and Deformed wing virus genomes. Israeli colonies showed high titers of IAPV and a conserved RNAi-pattern of matching the viral genome. That was also observed in sample analysis from colonies experimentally infected with IAPV. Our results suggest that CCD-colonies set out a siRNA response that is specific against predominant viruses associated with colony losses.

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JF - Virology

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Characterization of viral siRNA populations in honey bee colony collapse disorder

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