TY - JOUR
T1 - Characterization of viscoelastic properties of adsorbed biomolecules and biomolecular assemblies with high frequency micromachined quartz resonators
AU - Kao, Ping
AU - Allara, David
AU - Tadigadapa, Srinivas
N1 - Funding Information:
The authors acknowledge partial financial support from the NSF funded PSU Center for Nanoscale Science (MRSEC DMR-0080019) and the use of facilities at the PSU Site of the NSF NNIN under Agreement 0335765 is acknowledged. Kiron Mateti is acknowledged for Fig. 1 .
Funding Information:
Srinivas Tadigadapa is an Associate Professor of Electrical Engineering at the Pennsylvania State University. He obtained his MS from Indian Institute of Technology, Chennai and PhD from the University of Cambridge, UK in 1994. During 1996–2000 he was the Vice President of Manufacturing at Integrated Sensing Systems Inc., and was involved with the design, fabrication, packaging, reliability, and manufacturing of silicon microsystems. His research interests include microsystems and exploring phenomenon at the micro–nano interface. He has been a research fellow at the University of Karlsruhe, Germany and a Visiting Professor at Otto von Guericke University, Magdeburg, Germany, and University College, Cork, Ireland. He has been the recipient of the Alexander von Humboldt fellowship in Germany and the Walton Fellowship by the Science Foundation of Ireland. He is currently serving on the editorial boards of Institute of Physics Journal Measurement Science and Technology, and the SPIE Journal of Micro/Nanolithography, MEMS, and MOEMS.
PY - 2009/11/5
Y1 - 2009/11/5
N2 - Micromachined quartz crystal resonator arrays operating in the 66-69 MHz fundamental mode range were tested for their ability to provide high sensitivity to mass loading and viscoelastic properties of biomolecules and biomolecular assemblies. Calibrations using viscous water-glycerol mixtures give the expected linear dependence on the square root of the density-viscosity product. Sequential adsorption of avidin layers interspersed with dithiobis(sulfosuccinimidylpropionate) (DTSSP) or biotynilated bovine albumin (BBA) cross-linker layers provided thick and planar viscoelastic layers for testing. The data reveal a high Q-factor sensitivity and the observed complex impedance changes could be accounted for using a layer dependent, variable viscosity model treated with continuum mechanics approach. The best interpretation of the frequency and Q-factor changes indicates that the layer material properties are most likely dominated by the frictional effects arising from the inter-linking molecules between the layers. These results show the ability of these high frequency micro resonators as an incisive tool for analyzing both static loading and dynamic viscoelastic properties of bimolecular adsorbates.
AB - Micromachined quartz crystal resonator arrays operating in the 66-69 MHz fundamental mode range were tested for their ability to provide high sensitivity to mass loading and viscoelastic properties of biomolecules and biomolecular assemblies. Calibrations using viscous water-glycerol mixtures give the expected linear dependence on the square root of the density-viscosity product. Sequential adsorption of avidin layers interspersed with dithiobis(sulfosuccinimidylpropionate) (DTSSP) or biotynilated bovine albumin (BBA) cross-linker layers provided thick and planar viscoelastic layers for testing. The data reveal a high Q-factor sensitivity and the observed complex impedance changes could be accounted for using a layer dependent, variable viscosity model treated with continuum mechanics approach. The best interpretation of the frequency and Q-factor changes indicates that the layer material properties are most likely dominated by the frictional effects arising from the inter-linking molecules between the layers. These results show the ability of these high frequency micro resonators as an incisive tool for analyzing both static loading and dynamic viscoelastic properties of bimolecular adsorbates.
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U2 - 10.1016/j.snb.2009.01.046
DO - 10.1016/j.snb.2009.01.046
M3 - Article
AN - SCOPUS:71749105136
SN - 0925-4005
VL - 142
SP - 406
EP - 411
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 2
ER -