Characterizing in situ Glycerophospholipids with SIMS and MALDI Methodologies

M. K. Passarelli; N. Winograd

doi:10.1002/sia.3529

Characterizing in situ Glycerophospholipids with SIMS and MALDI Methodologies

M. K. Passarelli
, N. Winograd

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The dual functionality of a C₆₀⁺-Q-Star hybrid instrument allows for the examination of complex lipid profiles with both MALDI and SIMS methodologies.1 The difficulties associated with characterizing lipids in situ-isobaric interference and salt contamination-are discussed. The inherent advantages of both methodologies were used to deconvolute complex lipid spectra and establish characteristic fragmentation pathways. The high lateral resolution of SIMS allows for single cell images of aplysia californica neurons, while the established protocols were utilized to identify a major lipid component.

Original language	English (US)
Pages (from-to)	269-271
Number of pages	3
Journal	Surface and Interface Analysis
Volume	43
Issue number	1-2
DOIs	https://doi.org/10.1002/sia.3529
State	Published - Jan 2011

All Science Journal Classification (ASJC) codes

General Chemistry
Condensed Matter Physics
Surfaces and Interfaces
Surfaces, Coatings and Films
Materials Chemistry

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10.1002/sia.3529

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abstract = "The dual functionality of a C60+-Q-Star hybrid instrument allows for the examination of complex lipid profiles with both MALDI and SIMS methodologies.1 The difficulties associated with characterizing lipids in situ-isobaric interference and salt contamination-are discussed. The inherent advantages of both methodologies were used to deconvolute complex lipid spectra and establish characteristic fragmentation pathways. The high lateral resolution of SIMS allows for single cell images of aplysia californica neurons, while the established protocols were utilized to identify a major lipid component.",

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AB - The dual functionality of a C60+-Q-Star hybrid instrument allows for the examination of complex lipid profiles with both MALDI and SIMS methodologies.1 The difficulties associated with characterizing lipids in situ-isobaric interference and salt contamination-are discussed. The inherent advantages of both methodologies were used to deconvolute complex lipid spectra and establish characteristic fragmentation pathways. The high lateral resolution of SIMS allows for single cell images of aplysia californica neurons, while the established protocols were utilized to identify a major lipid component.

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JO - Surface and Interface Analysis

JF - Surface and Interface Analysis

IS - 1-2

ER -

Characterizing in situ Glycerophospholipids with SIMS and MALDI Methodologies

Abstract

All Science Journal Classification (ASJC) codes

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