Characterizing the Catecholamine Content of Single Mammalian Vesicles by Collision-Adsorption Events at an Electrode

Johan Dunevall; Hoda Fathali; Neda Najafinobar; Jelena Lovric; Joakim Wigström; Ann Sofie Cans; Andrew G. Ewing

doi:10.1021/ja512972f

Characterizing the Catecholamine Content of Single Mammalian Vesicles by Collision-Adsorption Events at an Electrode

Johan Dunevall
, Hoda Fathali
, Neda Najafinobar
, Jelena Lovric
, Joakim Wigström
, Ann Sofie Cans
, Andrew G. Ewing

Chemistry

Research output: Contribution to journal › Article › peer-review

175 Scopus citations

Abstract

We present the electrochemical response to single adrenal chromaffin vesicles filled with catecholamine hormones as they are adsorbed and rupture on a 33 μm diameter disk-shaped carbon electrode. The vesicles adsorb onto the electrode surface and sequentially spread out over the electrode surface, trapping their contents against the electrode. These contents are then oxidized, and a current (or amperometric) peak results from each vesicle that bursts. A large number of current transients associated with rupture of single vesicles (86%) are observed under the experimental conditions used, allowing us to quantify the vesicular catecholamine content.

Original language	English (US)
Pages (from-to)	4344-4346
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	137
Issue number	13
DOIs	https://doi.org/10.1021/ja512972f
State	Published - Apr 8 2015

All Science Journal Classification (ASJC) codes

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja512972f

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title = "Characterizing the Catecholamine Content of Single Mammalian Vesicles by Collision-Adsorption Events at an Electrode",

abstract = "We present the electrochemical response to single adrenal chromaffin vesicles filled with catecholamine hormones as they are adsorbed and rupture on a 33 μm diameter disk-shaped carbon electrode. The vesicles adsorb onto the electrode surface and sequentially spread out over the electrode surface, trapping their contents against the electrode. These contents are then oxidized, and a current (or amperometric) peak results from each vesicle that bursts. A large number of current transients associated with rupture of single vesicles (86\%) are observed under the experimental conditions used, allowing us to quantify the vesicular catecholamine content.",

author = "Johan Dunevall and Hoda Fathali and Neda Najafinobar and Jelena Lovric and Joakim Wigstr{\"o}m and Cans, \{Ann Sofie\} and Ewing, \{Andrew G.\}",

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AU - Dunevall, Johan

AU - Fathali, Hoda

AU - Najafinobar, Neda

AU - Lovric, Jelena

AU - Wigström, Joakim

AU - Cans, Ann Sofie

AU - Ewing, Andrew G.

PY - 2015/4/8

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N2 - We present the electrochemical response to single adrenal chromaffin vesicles filled with catecholamine hormones as they are adsorbed and rupture on a 33 μm diameter disk-shaped carbon electrode. The vesicles adsorb onto the electrode surface and sequentially spread out over the electrode surface, trapping their contents against the electrode. These contents are then oxidized, and a current (or amperometric) peak results from each vesicle that bursts. A large number of current transients associated with rupture of single vesicles (86%) are observed under the experimental conditions used, allowing us to quantify the vesicular catecholamine content.

AB - We present the electrochemical response to single adrenal chromaffin vesicles filled with catecholamine hormones as they are adsorbed and rupture on a 33 μm diameter disk-shaped carbon electrode. The vesicles adsorb onto the electrode surface and sequentially spread out over the electrode surface, trapping their contents against the electrode. These contents are then oxidized, and a current (or amperometric) peak results from each vesicle that bursts. A large number of current transients associated with rupture of single vesicles (86%) are observed under the experimental conditions used, allowing us to quantify the vesicular catecholamine content.

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Characterizing the Catecholamine Content of Single Mammalian Vesicles by Collision-Adsorption Events at an Electrode

Abstract

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