TY - JOUR
T1 - Chemical profiles and monitoring dynamics at an individual nerve cell in Planorbis corneus with electrochemical detection
AU - Anderson, Brian B.
AU - Ewing, Andrew G.
N1 - Funding Information:
Support from the National Science Foundation and the National Institutes of Health is gratefully acknowledged. We would also like to thank all our coworkers for the work referenced herein.
PY - 1999/2
Y1 - 1999/2
N2 - The identified dopamine cell of Planorbis corneus is described as a model system to study neurotransmitter storage and dynamics. Techniques developed with this model system include capillary electrophoresis with electrochemical detection and microelectrochemistry at single cells. These techniques provide a powerful combination to examine single cell neurochemistry. Whole cell and cytoplasmic dopamine concentrations have been quantified with capillary electrophoresis. Additionally, this technique has been used to profile amino acids and to quantify two compartments of neurotransmitter in a single cell. Individual exocytosis events have been monitored at the cell body of the dopamine cell of P. corneus with microelectrodes. In this case, two different types of vesicles have been identified based on the amount of transmitter released. The psychostimulant, amphetamine, has been shown to selectively affect the amount of dopamine in these vesicles with lower to higher doses affecting the larger to smaller vesicle types, respectively. Microelectrochemistry at single nerve cells has also been used to demonstrate reverse transport of dopamine across the cell membrane and to suggest a role of this process in the molecular mechanism of amphetamine. Copyright (C) 1999 Elsevier Science B.V.
AB - The identified dopamine cell of Planorbis corneus is described as a model system to study neurotransmitter storage and dynamics. Techniques developed with this model system include capillary electrophoresis with electrochemical detection and microelectrochemistry at single cells. These techniques provide a powerful combination to examine single cell neurochemistry. Whole cell and cytoplasmic dopamine concentrations have been quantified with capillary electrophoresis. Additionally, this technique has been used to profile amino acids and to quantify two compartments of neurotransmitter in a single cell. Individual exocytosis events have been monitored at the cell body of the dopamine cell of P. corneus with microelectrodes. In this case, two different types of vesicles have been identified based on the amount of transmitter released. The psychostimulant, amphetamine, has been shown to selectively affect the amount of dopamine in these vesicles with lower to higher doses affecting the larger to smaller vesicle types, respectively. Microelectrochemistry at single nerve cells has also been used to demonstrate reverse transport of dopamine across the cell membrane and to suggest a role of this process in the molecular mechanism of amphetamine. Copyright (C) 1999 Elsevier Science B.V.
UR - http://www.scopus.com/inward/record.url?scp=0032922401&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032922401&partnerID=8YFLogxK
U2 - 10.1016/S0731-7085(98)00088-0
DO - 10.1016/S0731-7085(98)00088-0
M3 - Article
C2 - 10698565
AN - SCOPUS:0032922401
SN - 0731-7085
VL - 19
SP - 15
EP - 32
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
IS - 1-2
ER -