Chemical Synthesis of a Bisphosphorylated Mannose-6-Phosphate N-Glycan and its Facile Monoconjugation with Human Carbonic Anhydrase II for in vivo Fluorescence Imaging

Yunpeng Liu; Yan Mei Chan; Jianhui Wu; Chen Chen; Alan Benesi; Jing Hu; Yanming Wang; Gong Chen

doi:10.1002/cbic.201000785

Chemical Synthesis of a Bisphosphorylated Mannose-6-Phosphate N-Glycan and its Facile Monoconjugation with Human Carbonic Anhydrase II for in vivo Fluorescence Imaging

Yunpeng Liu, Yan Mei Chan, Jianhui Wu, Chen Chen, Alan Benesi, Jing Hu, Yanming Wang, Gong Chen

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Red-ily made: The first chemical synthesis of a fully elaborated bisphosphorylated triantennary M6P N-glycan was achieved through a highly efficient late-stage phosphorylation strategy. A human carbonic anhydrase II-based fluorescently tagged neoglycoprotein platform was successfully constructed in order to evaluate the M6P N-glycan-directed protein internalization process in cell-based assays by confocal fluorescence imaging.

Original language	English (US)
Pages (from-to)	685-690
Number of pages	6
Journal	ChemBioChem
Volume	12
Issue number	5
DOIs	https://doi.org/10.1002/cbic.201000785
State	Published - Mar 21 2011

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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AU - Chan, Yan Mei

AU - Wu, Jianhui

AU - Chen, Chen

AU - Benesi, Alan

AU - Hu, Jing

AU - Wang, Yanming

AU - Chen, Gong

PY - 2011/3/21

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Chemical Synthesis of a Bisphosphorylated Mannose-6-Phosphate N-Glycan and its Facile Monoconjugation with Human Carbonic Anhydrase II for in vivo Fluorescence Imaging

Abstract

All Science Journal Classification (ASJC) codes

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