Chemical Synthesis of a Bisphosphorylated Mannose-6-Phosphate N-Glycan and its Facile Monoconjugation with Human Carbonic Anhydrase II for in vivo Fluorescence Imaging

Yunpeng Liu; Yan Mei Chan; Jianhui Wu; Chen Chen; Alan Benesi; Jing Hu; Yanming Wang; Gong Chen

doi:10.1002/cbic.201000785

Chemical Synthesis of a Bisphosphorylated Mannose-6-Phosphate N-Glycan and its Facile Monoconjugation with Human Carbonic Anhydrase II for in vivo Fluorescence Imaging

Yunpeng Liu
, Yan Mei Chan
, Jianhui Wu
, Chen Chen
, Alan Benesi
, Jing Hu
, Yanming Wang
, Gong Chen

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Red-ily made: The first chemical synthesis of a fully elaborated bisphosphorylated triantennary M6P N-glycan was achieved through a highly efficient late-stage phosphorylation strategy. A human carbonic anhydrase II-based fluorescently tagged neoglycoprotein platform was successfully constructed in order to evaluate the M6P N-glycan-directed protein internalization process in cell-based assays by confocal fluorescence imaging.

Original language	English (US)
Pages (from-to)	685-690
Number of pages	6
Journal	ChemBioChem
Volume	12
Issue number	5
DOIs	https://doi.org/10.1002/cbic.201000785
State	Published - Mar 21 2011

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.201000785

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abstract = "Red-ily made: The first chemical synthesis of a fully elaborated bisphosphorylated triantennary M6P N-glycan was achieved through a highly efficient late-stage phosphorylation strategy. A human carbonic anhydrase II-based fluorescently tagged neoglycoprotein platform was successfully constructed in order to evaluate the M6P N-glycan-directed protein internalization process in cell-based assays by confocal fluorescence imaging.",

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AU - Liu, Yunpeng

AU - Chan, Yan Mei

AU - Wu, Jianhui

AU - Chen, Chen

AU - Benesi, Alan

AU - Hu, Jing

AU - Wang, Yanming

AU - Chen, Gong

PY - 2011/3/21

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Chemical Synthesis of a Bisphosphorylated Mannose-6-Phosphate N-Glycan and its Facile Monoconjugation with Human Carbonic Anhydrase II for in vivo Fluorescence Imaging

Abstract

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