Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells

Xiaoping Bao; Xiaojun Lian; Kaitlin K. Dunn; Mengxuan Shi; Tianxiao Han; Tongcheng Qian; Vijesh J. Bhute; Scott G. Canfield; Sean P. Palecek

doi:10.1016/j.scr.2015.05.004

Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells

Xiaoping Bao, Xiaojun Lian, Kaitlin K. Dunn, Mengxuan Shi, Tianxiao Han, Tongcheng Qian, Vijesh J. Bhute, Scott G. Canfield, Sean P. Palecek

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

Human pluripotent stem cell (hPSC)-derived endothelial cells and their progenitors are important for vascular research and therapeutic revascularization. Here, we report a completely defined endothelial progenitor differentiation platform that uses a minimalistic medium consisting of Dulbecco's modified eagle medium and ascorbic acid, lacking of albumin and growth factors. Following hPSC treatment with a GSK-3β inhibitor and culture in this medium, this protocol generates more than 30% multipotent CD34. + CD31. + endothelial progenitors that can be purified to >. 95% CD34. + cells via magnetic activated cell sorting (MACS). These CD34. + progenitors are capable of differentiating into endothelial cells in serum-free inductive media. These hPSC-derived endothelial cells express key endothelial markers including CD31, VE-cadherin, and von Willebrand factor (vWF), exhibit endothelial-specific phenotypes and functions including tube formation and acetylated low-density lipoprotein (Ac-LDL) uptake. This fully defined platform should facilitate production of proliferative, xeno-free endothelial progenitor cells for both research and clinical applications.

Original language	English (US)
Pages (from-to)	122-129
Number of pages	8
Journal	Stem Cell Research
Volume	15
Issue number	1
DOIs	https://doi.org/10.1016/j.scr.2015.05.004
State	Published - Jul 1 2015

All Science Journal Classification (ASJC) codes

Developmental Biology
Cell Biology

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10.1016/j.scr.2015.05.004

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title = "Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells",

abstract = "Human pluripotent stem cell (hPSC)-derived endothelial cells and their progenitors are important for vascular research and therapeutic revascularization. Here, we report a completely defined endothelial progenitor differentiation platform that uses a minimalistic medium consisting of Dulbecco's modified eagle medium and ascorbic acid, lacking of albumin and growth factors. Following hPSC treatment with a GSK-3β inhibitor and culture in this medium, this protocol generates more than 30% multipotent CD34. + CD31. + endothelial progenitors that can be purified to >. 95% CD34. + cells via magnetic activated cell sorting (MACS). These CD34. + progenitors are capable of differentiating into endothelial cells in serum-free inductive media. These hPSC-derived endothelial cells express key endothelial markers including CD31, VE-cadherin, and von Willebrand factor (vWF), exhibit endothelial-specific phenotypes and functions including tube formation and acetylated low-density lipoprotein (Ac-LDL) uptake. This fully defined platform should facilitate production of proliferative, xeno-free endothelial progenitor cells for both research and clinical applications.",

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AU - Lian, Xiaojun

AU - Dunn, Kaitlin K.

AU - Shi, Mengxuan

AU - Han, Tianxiao

AU - Qian, Tongcheng

AU - Bhute, Vijesh J.

AU - Canfield, Scott G.

AU - Palecek, Sean P.

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Human pluripotent stem cell (hPSC)-derived endothelial cells and their progenitors are important for vascular research and therapeutic revascularization. Here, we report a completely defined endothelial progenitor differentiation platform that uses a minimalistic medium consisting of Dulbecco's modified eagle medium and ascorbic acid, lacking of albumin and growth factors. Following hPSC treatment with a GSK-3β inhibitor and culture in this medium, this protocol generates more than 30% multipotent CD34. + CD31. + endothelial progenitors that can be purified to >. 95% CD34. + cells via magnetic activated cell sorting (MACS). These CD34. + progenitors are capable of differentiating into endothelial cells in serum-free inductive media. These hPSC-derived endothelial cells express key endothelial markers including CD31, VE-cadherin, and von Willebrand factor (vWF), exhibit endothelial-specific phenotypes and functions including tube formation and acetylated low-density lipoprotein (Ac-LDL) uptake. This fully defined platform should facilitate production of proliferative, xeno-free endothelial progenitor cells for both research and clinical applications.

AB - Human pluripotent stem cell (hPSC)-derived endothelial cells and their progenitors are important for vascular research and therapeutic revascularization. Here, we report a completely defined endothelial progenitor differentiation platform that uses a minimalistic medium consisting of Dulbecco's modified eagle medium and ascorbic acid, lacking of albumin and growth factors. Following hPSC treatment with a GSK-3β inhibitor and culture in this medium, this protocol generates more than 30% multipotent CD34. + CD31. + endothelial progenitors that can be purified to >. 95% CD34. + cells via magnetic activated cell sorting (MACS). These CD34. + progenitors are capable of differentiating into endothelial cells in serum-free inductive media. These hPSC-derived endothelial cells express key endothelial markers including CD31, VE-cadherin, and von Willebrand factor (vWF), exhibit endothelial-specific phenotypes and functions including tube formation and acetylated low-density lipoprotein (Ac-LDL) uptake. This fully defined platform should facilitate production of proliferative, xeno-free endothelial progenitor cells for both research and clinical applications.

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Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells

Abstract

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