Chemically defined generation of human definitive hematopoietic stem and progenitor cells

Juhyung Jung; Yun Chang; Gyuhyung Jin; Qing Deng; Xiaojun Lance Lian; Xiaoping Bao

doi:10.1016/j.xpro.2022.101953

Chemically defined generation of human definitive hematopoietic stem and progenitor cells

Juhyung Jung, Yun Chang, Gyuhyung Jin, Qing Deng, Xiaojun Lance Lian, Xiaoping Bao

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3 Scopus citations

Abstract

Here, we present a protocol to efficiently direct human pluripotent stem cells (hPSCs) into hematopoietic stem and progenitor cells (HSPCs) under a chemically defined, albumin-free system. We describe the induction of aorta-gonad-mesonephros-like hematopoiesis from hPSCs into SOX17+ hemogenic endothelium and then into CD34+CD45+ HSPCs via application of Wnt activator and TGFβ inhibitor, respectively. The generated HSPCs, characterized by flow cytometry and colony-forming unit assay, express definitive hematopoiesis markers and exhibit multilineage differentiation potential and the capacity to expand. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022a, 2022b).^1,2

Original language	English (US)
Article number	101953
Journal	STAR Protocols
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2022.101953
State	Published - Mar 17 2023

All Science Journal Classification (ASJC) codes

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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title = "Chemically defined generation of human definitive hematopoietic stem and progenitor cells",

abstract = "Here, we present a protocol to efficiently direct human pluripotent stem cells (hPSCs) into hematopoietic stem and progenitor cells (HSPCs) under a chemically defined, albumin-free system. We describe the induction of aorta-gonad-mesonephros-like hematopoiesis from hPSCs into SOX17+ hemogenic endothelium and then into CD34+CD45+ HSPCs via application of Wnt activator and TGFβ inhibitor, respectively. The generated HSPCs, characterized by flow cytometry and colony-forming unit assay, express definitive hematopoiesis markers and exhibit multilineage differentiation potential and the capacity to expand. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022a, 2022b).1,2",

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AU - Jung, Juhyung

AU - Chang, Yun

AU - Jin, Gyuhyung

AU - Deng, Qing

AU - Lian, Xiaojun Lance

AU - Bao, Xiaoping

PY - 2023/3/17

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AB - Here, we present a protocol to efficiently direct human pluripotent stem cells (hPSCs) into hematopoietic stem and progenitor cells (HSPCs) under a chemically defined, albumin-free system. We describe the induction of aorta-gonad-mesonephros-like hematopoiesis from hPSCs into SOX17+ hemogenic endothelium and then into CD34+CD45+ HSPCs via application of Wnt activator and TGFβ inhibitor, respectively. The generated HSPCs, characterized by flow cytometry and colony-forming unit assay, express definitive hematopoiesis markers and exhibit multilineage differentiation potential and the capacity to expand. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022a, 2022b).1,2

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Chemically defined generation of human definitive hematopoietic stem and progenitor cells

Abstract

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