Chemically defined generation of human definitive hematopoietic stem and progenitor cells

Juhyung Jung, Yun Chang, Gyuhyung Jin, Qing Deng, Xiaojun Lance Lian, Xiaoping Bao

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Here, we present a protocol to efficiently direct human pluripotent stem cells (hPSCs) into hematopoietic stem and progenitor cells (HSPCs) under a chemically defined, albumin-free system. We describe the induction of aorta-gonad-mesonephros-like hematopoiesis from hPSCs into SOX17+ hemogenic endothelium and then into CD34+CD45+ HSPCs via application of Wnt activator and TGFβ inhibitor, respectively. The generated HSPCs, characterized by flow cytometry and colony-forming unit assay, express definitive hematopoiesis markers and exhibit multilineage differentiation potential and the capacity to expand. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022a, 2022b).1,2

Original languageEnglish (US)
Article number101953
JournalSTAR Protocols
Issue number1
StatePublished - Mar 17 2023

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


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