Chronic optical access through a polished and reinforced thinned skull

Patrick James Drew; Andy Y. Shih; Jonathan D. Driscoll; Per Magne Knutsen; Pablo Blinder; Dimitrios Davalos; Katerina Akassoglou; Philbert S. Tsai; David Kleinfeld

doi:10.1038/nmeth.1530

Chronic optical access through a polished and reinforced thinned skull

Patrick James Drew, Andy Y. Shih, Jonathan D. Driscoll, Per Magne Knutsen, Pablo Blinder, Dimitrios Davalos, Katerina Akassoglou, Philbert S. Tsai, David Kleinfeld

Research output: Contribution to journal › Article › peer-review

326 Scopus citations

Abstract

We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without causing brain inflammation. This enabled us to repeatedly image blood flow in cortical capillaries of awake mice and determine long-range correlations in speed. We also repeatedly imaged dendritic spines, microglia and angioarchitecture, as well as used illumination to drive motor output via optogenetics and induce microstrokes via photosensitizers.

Original language	English (US)
Pages (from-to)	981-984
Number of pages	4
Journal	Nature methods
Volume	7
Issue number	12
DOIs	https://doi.org/10.1038/nmeth.1530
State	Published - Dec 2010

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.1530

Cite this

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title = "Chronic optical access through a polished and reinforced thinned skull",

abstract = "We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without causing brain inflammation. This enabled us to repeatedly image blood flow in cortical capillaries of awake mice and determine long-range correlations in speed. We also repeatedly imaged dendritic spines, microglia and angioarchitecture, as well as used illumination to drive motor output via optogenetics and induce microstrokes via photosensitizers.",

author = "Drew, {Patrick James} and Shih, {Andy Y.} and Driscoll, {Jonathan D.} and Knutsen, {Per Magne} and Pablo Blinder and Dimitrios Davalos and Katerina Akassoglou and Tsai, {Philbert S.} and David Kleinfeld",

note = "Funding Information: We thank B. Friedman, C. Portera-Cailliau and K. Svoboda for critical comments on an early version of the manuscript, M. Fuentes and C. Petersen for advice on head fixation, D.N. Hill for discussions on data analysis, J. Lee and K. Yang for help with animal husbandry, J.D. Moore for discussions on optical stimulation, and S. Tayman for a gift of tin oxide. This work was supported by grants from the",

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AU - Shih, Andy Y.

AU - Driscoll, Jonathan D.

AU - Knutsen, Per Magne

AU - Blinder, Pablo

AU - Davalos, Dimitrios

AU - Akassoglou, Katerina

AU - Tsai, Philbert S.

AU - Kleinfeld, David

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Chronic optical access through a polished and reinforced thinned skull

Abstract

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