TY - JOUR
T1 - Circulating rotavirus-specific Antibody-Secreting Cells (ASCs) predict the presence of rotavirus-specific ASCs in the human small intestinal lamina propria
AU - Brown, Kurt A.
AU - Kriss, Jennifer A.
AU - Moser, Charlotte A.
AU - Wenner, William J.
AU - Offit, Paul A.
N1 - Funding Information:
Received 24 January 2000; revised 4 May 2000; electronically published 5 September 2000 Written informed consent was obtained from patients, parents, or guardians. Human experimentation guidelines were followed according to the Committee for Protection of Human Subjects Institutional Review Board (IRB) at Children’s Hospital of Philadelphia (IRB no. 1997-10-1369). Financial support: National Institutes of Health (KO8-02383 to K.A.B); Merck and Co. a Present affiliation: Merck and Co., Bluebell, Pennsylvania. Reprints or correspondence: Dr. Kurt A. Brown, Division of Gastroenterology and Nutrition, Children’s Hospital of Philadelphia, 34th St. and Civic Center Blvd., Philadelphia, PA 19104 ([email protected]).
PY - 2000
Y1 - 2000
N2 - Rotaviruses are the most important cause of infectious diarrhea in children throughout the world. Protection is most likely mediated by small-intestinal virus-specific IgA. However, neither fecal nor serum virus-specific IgA clearly correlates with protection against challenge. The capacity of rotavirus-specific antibodies and rotavirus-specific antibody-secreting cells (ASCs) in the circulation to predict the presence of ASCs in the intestines of children was evaluated. Mononuclear cells from intestinal biopsy samples and blood from 21 children were enriched for CD38, a marker of terminally differentiated B cells, and evaluated for the presence of virus-specific and total IgA- and IgG-secreting cells, by ELISPOT assay. Serum virus-specific IgA and IgG levels were determined by ELISA. The ratio of virus-specific to total IgA-secreting cells in the blood correlated with that found in the small, but not large, intestine. In contrast, serum rotavirus-specific IgA correlated less well with the presence of virus-specific ASCs in the small intestine.
AB - Rotaviruses are the most important cause of infectious diarrhea in children throughout the world. Protection is most likely mediated by small-intestinal virus-specific IgA. However, neither fecal nor serum virus-specific IgA clearly correlates with protection against challenge. The capacity of rotavirus-specific antibodies and rotavirus-specific antibody-secreting cells (ASCs) in the circulation to predict the presence of ASCs in the intestines of children was evaluated. Mononuclear cells from intestinal biopsy samples and blood from 21 children were enriched for CD38, a marker of terminally differentiated B cells, and evaluated for the presence of virus-specific and total IgA- and IgG-secreting cells, by ELISPOT assay. Serum virus-specific IgA and IgG levels were determined by ELISA. The ratio of virus-specific to total IgA-secreting cells in the blood correlated with that found in the small, but not large, intestine. In contrast, serum rotavirus-specific IgA correlated less well with the presence of virus-specific ASCs in the small intestine.
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U2 - 10.1086/315808
DO - 10.1086/315808
M3 - Article
C2 - 10979897
AN - SCOPUS:0033805375
SN - 0022-1899
VL - 182
SP - 1039
EP - 1043
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -
