TY - JOUR
T1 - Cis-acting, recombination-stimulating activity in a fragment of the ribosomal DNA of S. cerevisiae
AU - Keil, Ralph L.
AU - Shirleen Roeder, G.
N1 - Funding Information:
We thank James E. Haber, Richard G. Harrison, Eric J. Lambie, and Jack W. Szostak for their helpful comments on the manuscript. R. L. K. was supported by a postdoctoral fellowship from the American Cancer Society (PF-2088). This work was funded by Public Health Service grant GM28904 from the National institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
PY - 1984/12
Y1 - 1984/12
N2 - Special mechanisms for stimulating recombination among the nearly identical repeat units of certain multigene families may exist in order to maintain their sequence homogeneity. We have found evidence for such a recombination-stimulating activity in the tandemly repeated ribosomal RNA genes of yeast. A fragment of the yeast ribosomal DNA (rDNA), containing the 5S gene, nontranscribed spacer DNA, and part of the 25S gene, causes a localized stimulation of recombination when inserted at novel locations in the yeast genome. The rDNA fragment stimulates both interchromosomal and intrachromosomal mitotic recombination but not meiotic recombination. To stimulate mitotic recombination, the fragment must act on both copies of the recombining gene. Furthermore, the rDNA fragment stimulates exchange only when inserted with the 5S gene proximal to, and the 25S gene distal to, the recombining alleles.
AB - Special mechanisms for stimulating recombination among the nearly identical repeat units of certain multigene families may exist in order to maintain their sequence homogeneity. We have found evidence for such a recombination-stimulating activity in the tandemly repeated ribosomal RNA genes of yeast. A fragment of the yeast ribosomal DNA (rDNA), containing the 5S gene, nontranscribed spacer DNA, and part of the 25S gene, causes a localized stimulation of recombination when inserted at novel locations in the yeast genome. The rDNA fragment stimulates both interchromosomal and intrachromosomal mitotic recombination but not meiotic recombination. To stimulate mitotic recombination, the fragment must act on both copies of the recombining gene. Furthermore, the rDNA fragment stimulates exchange only when inserted with the 5S gene proximal to, and the 25S gene distal to, the recombining alleles.
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U2 - 10.1016/0092-8674(84)90016-3
DO - 10.1016/0092-8674(84)90016-3
M3 - Article
C2 - 6094015
AN - SCOPUS:0021750302
SN - 0092-8674
VL - 39
SP - 377
EP - 386
JO - Cell
JF - Cell
IS - 2 PART 1
ER -