Clonally variant gene families in Plasmodium falciparum share a common activation factor

Call A. Howitt, Daniel Willnski, Manuel Llinás, Thomas J. Templeton, Ron Dzlkowski, Kirk W. Deitsch

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


The genome of the malaria parasite Plasmodium falciparum contains several multicopy gene families, including var, rifin, stevor and Pfmc-2TM. These gene families undergo expression switching and appear to play a role in antigenic variation. It has recently been shown that forcing parasites to express high copy numbers of transcriptionally active, episomal var promoters led to gradual downregulation and eventual silencing of the entire var gene family, suggesting that a limiting titratable factor plays a role in var gene activation. Through similar experiments using rifin, stevor or Pfmc-2TMepisomal promoters we show that promoter titration can be used as a general method to downregulate multicopy gene families in P. falciparum. Additionally, we show that promoter titration with var, rifin, stevor or Pfmc-2TM episomal promoters results in downregulation of expression not only of the family to which the episomal promoter belongs, but also members of the other gene families, suggesting that the var-specific titratable factor previously described is shared by all four families. Further, transcriptionally active promoters from different families colocalize within the same subnuclear expression site, indicating that the role that nuclear architecture plays in var gene regulation also likely applies to the other multicopy gene families of P. falciparum.

Original languageEnglish (US)
Pages (from-to)1171-1185
Number of pages15
JournalMolecular Microbiology
Issue number6
StatePublished - Sep 2009

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology


Dive into the research topics of 'Clonally variant gene families in Plasmodium falciparum share a common activation factor'. Together they form a unique fingerprint.

Cite this