Cloning and characterization of 40S ribosomal protein S4 gene from Culex pipiens pallens

Xiaobang Hu; Weijie Wang; Donghui Zhang; Jianhua Jiao; Wenbin Tan; Yan Sun; Lei Ma; Changliang Zhu

doi:10.1016/j.cbpb.2006.11.011

Cloning and characterization of 40S ribosomal protein S4 gene from Culex pipiens pallens

Xiaobang Hu, Weijie Wang, Donghui Zhang, Jianhua Jiao, Wenbin Tan, Yan Sun, Lei Ma, Changliang Zhu

Department of Pathology and Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The 40S ribosomal protein S4 gene (RPS4) has been cloned from Culex pipiens pallens. An open reading frame (ORF) of 789 bp was found to encode a putative 262 amino acid protein. The deduced amino acid sequence shares 96% and 91% identity with RPS4 genes from Aedes and Anopheles respectively. Transcript expression of RPS4 was determined by real-time PCR in all life stages of deltamethrin-susceptible and -resistant strains. The results demonstrated that this gene is expressed at all developmental stages. Meanwhile, in pupae and adults, RPS4 is overexpressed in deltamethrin-resistant strain than in -susceptible strain. Our data for the first time suggests that increased expression of the RPS4 gene may play some role in the development of deltamethrin resistance in C. pipiens pallens.

Original language	English (US)
Pages (from-to)	265-270
Number of pages	6
Journal	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	146
Issue number	2
DOIs	https://doi.org/10.1016/j.cbpb.2006.11.011
State	Published - Feb 2007

All Science Journal Classification (ASJC) codes

Biochemistry
Physiology
Molecular Biology

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10.1016/j.cbpb.2006.11.011

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title = "Cloning and characterization of 40S ribosomal protein S4 gene from Culex pipiens pallens",

abstract = "The 40S ribosomal protein S4 gene (RPS4) has been cloned from Culex pipiens pallens. An open reading frame (ORF) of 789 bp was found to encode a putative 262 amino acid protein. The deduced amino acid sequence shares 96% and 91% identity with RPS4 genes from Aedes and Anopheles respectively. Transcript expression of RPS4 was determined by real-time PCR in all life stages of deltamethrin-susceptible and -resistant strains. The results demonstrated that this gene is expressed at all developmental stages. Meanwhile, in pupae and adults, RPS4 is overexpressed in deltamethrin-resistant strain than in -susceptible strain. Our data for the first time suggests that increased expression of the RPS4 gene may play some role in the development of deltamethrin resistance in C. pipiens pallens.",

author = "Xiaobang Hu and Weijie Wang and Donghui Zhang and Jianhua Jiao and Wenbin Tan and Yan Sun and Lei Ma and Changliang Zhu",

note = "Funding Information: We thank Dr. Jianming Li and Mrs. Jinmei Xu for their help about real-time PCR and data analysis. And we would like to thank the anonymous reviewers for their helpful comments and suggestions. This work was supported by the National Natural Science Foundation of China (No. 30170835 and 30371255) and the Natural Science Foundation of Jiangsu Province (No. BK2004147).",

year = "2007",

month = feb,

doi = "10.1016/j.cbpb.2006.11.011",

language = "English (US)",

volume = "146",

pages = "265--270",

journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",

issn = "1096-4959",

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T1 - Cloning and characterization of 40S ribosomal protein S4 gene from Culex pipiens pallens

AU - Hu, Xiaobang

AU - Wang, Weijie

AU - Zhang, Donghui

AU - Jiao, Jianhua

AU - Tan, Wenbin

AU - Sun, Yan

AU - Ma, Lei

AU - Zhu, Changliang

N1 - Funding Information: We thank Dr. Jianming Li and Mrs. Jinmei Xu for their help about real-time PCR and data analysis. And we would like to thank the anonymous reviewers for their helpful comments and suggestions. This work was supported by the National Natural Science Foundation of China (No. 30170835 and 30371255) and the Natural Science Foundation of Jiangsu Province (No. BK2004147).

PY - 2007/2

Y1 - 2007/2

N2 - The 40S ribosomal protein S4 gene (RPS4) has been cloned from Culex pipiens pallens. An open reading frame (ORF) of 789 bp was found to encode a putative 262 amino acid protein. The deduced amino acid sequence shares 96% and 91% identity with RPS4 genes from Aedes and Anopheles respectively. Transcript expression of RPS4 was determined by real-time PCR in all life stages of deltamethrin-susceptible and -resistant strains. The results demonstrated that this gene is expressed at all developmental stages. Meanwhile, in pupae and adults, RPS4 is overexpressed in deltamethrin-resistant strain than in -susceptible strain. Our data for the first time suggests that increased expression of the RPS4 gene may play some role in the development of deltamethrin resistance in C. pipiens pallens.

AB - The 40S ribosomal protein S4 gene (RPS4) has been cloned from Culex pipiens pallens. An open reading frame (ORF) of 789 bp was found to encode a putative 262 amino acid protein. The deduced amino acid sequence shares 96% and 91% identity with RPS4 genes from Aedes and Anopheles respectively. Transcript expression of RPS4 was determined by real-time PCR in all life stages of deltamethrin-susceptible and -resistant strains. The results demonstrated that this gene is expressed at all developmental stages. Meanwhile, in pupae and adults, RPS4 is overexpressed in deltamethrin-resistant strain than in -susceptible strain. Our data for the first time suggests that increased expression of the RPS4 gene may play some role in the development of deltamethrin resistance in C. pipiens pallens.

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JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

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Cloning and characterization of 40S ribosomal protein S4 gene from Culex pipiens pallens

Abstract

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