TY - JOUR
T1 - Cloning and characterization of NYD-OP7, a novel deltamethrin resistance associated gene from Culex pipiens pallens
AU - Hu, Xiaobang
AU - Sun, Yan
AU - Wang, Weijie
AU - Yang, Mingxia
AU - Sun, Lixin
AU - Tan, Wenbin
AU - Sun, Jing
AU - Qian, Jin
AU - Ma, Lei
AU - Zhang, Donghui
AU - Yan, Guiyun
AU - Zhu, Changliang
N1 - Funding Information:
We thank Dr. Grace Jones (Department of Biology, University of Kentucky, USA) for his generous gift of plasmid pIE1-neo. We thank Mrs. Jinmei Xu for her help about data analysis. We also thank the anonymous reviewers for their helpful comments and suggestions. This work was supported by the National Natural Science Foundation of China (Nos. 30170835 and 30371255), the Natural Science Foundation of Jiangsu Province (No. BK2004147) and the Academic Natural Science Foundation of Jiangsu Province (No. 03KJB180082).
PY - 2007/5
Y1 - 2007/5
N2 - One mosquito opsin gene, NYD-OP7, has been cloned from Culex pipiens pallens. An open reading frame (ORF) of 1116 bp was found to encode a putative 371 amino acids protein which exhibits high identity with opsins from Aedes and Anopheles mosquitoes. Transcript expression of NYD-OP7 was determined by real-time PCR in all life stages of deltamethrin-susceptible and -resistant strains of the Culex mosquito. The results demonstrated that this gene is expressed at all developmental stages, and it is expressed predominantly at the pupae and adult stages. Meanwhile, in pupae and adults, NYD-OP7 is overexpressed in deltamethrin-resistant strain than in -susceptible strain. Importantly, stable expression of NYD-OP7 in the mosquito C6/36 cells can confer moderate deltamethrin resistance. Our study provides the first direct evidence that increased expression of an opsin gene may play some role in the development of deltamethrin resistance in Cx. pipiens pallens.
AB - One mosquito opsin gene, NYD-OP7, has been cloned from Culex pipiens pallens. An open reading frame (ORF) of 1116 bp was found to encode a putative 371 amino acids protein which exhibits high identity with opsins from Aedes and Anopheles mosquitoes. Transcript expression of NYD-OP7 was determined by real-time PCR in all life stages of deltamethrin-susceptible and -resistant strains of the Culex mosquito. The results demonstrated that this gene is expressed at all developmental stages, and it is expressed predominantly at the pupae and adult stages. Meanwhile, in pupae and adults, NYD-OP7 is overexpressed in deltamethrin-resistant strain than in -susceptible strain. Importantly, stable expression of NYD-OP7 in the mosquito C6/36 cells can confer moderate deltamethrin resistance. Our study provides the first direct evidence that increased expression of an opsin gene may play some role in the development of deltamethrin resistance in Cx. pipiens pallens.
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U2 - 10.1016/j.pestbp.2006.09.004
DO - 10.1016/j.pestbp.2006.09.004
M3 - Article
AN - SCOPUS:33847358575
SN - 0048-3575
VL - 88
SP - 82
EP - 91
JO - Pesticide Biochemistry and Physiology
JF - Pesticide Biochemistry and Physiology
IS - 1
ER -