Cloning and overexpression of ribosomal protein L39 gene from deltamethrin-resistant Culex pipiens pallens

Wenbin Tan; Lixin Sun; Donghui Zhang; Jing Sun; Jin Qian; Xiaobang Hu; Weijie Wang; Yan Sun; Lei Ma; Changliang Zhu

doi:10.1016/j.exppara.2006.09.020

Cloning and overexpression of ribosomal protein L39 gene from deltamethrin-resistant Culex pipiens pallens

Wenbin Tan, Lixin Sun, Donghui Zhang, Jing Sun, Jin Qian, Xiaobang Hu, Weijie Wang, Yan Sun, Lei Ma, Changliang Zhu

Department of Pathology and Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The complete sequence of ribosomal protein L39 has been cloned from deltamethrin-resistant strain of Culex pipiens pallens (DR1 strain). Quantitative RT-PCR analysis indicated that the RPL39 transcription level was 23.4 times higher in DR1 strain than in susceptible strain at 4th instar larvae. The RPL39 expression was also found to be consistently higher throughout the life cycle of DR1 strain. A protein of predicted size 17 kDa has been detected by Western blotting in RPL39-transfected mosquito C6/36 cells. These RPL39-transfected cells also showed enhanced deltamethrin resistance compared to plasmid vector-transfected cells as determined by methyl tritiated thymidine (³H-TdR) incorporation. These results indicate that RPL39 is expressed at higher levels in DR1 strain, and may confer some insecticide resistance in Cx. pipiens pallens.

Original language	English (US)
Pages (from-to)	369-378
Number of pages	10
Journal	Experimental Parasitology
Volume	115
Issue number	4
DOIs	https://doi.org/10.1016/j.exppara.2006.09.020
State	Published - Apr 2007

All Science Journal Classification (ASJC) codes

Parasitology
Immunology
Infectious Diseases

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10.1016/j.exppara.2006.09.020

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title = "Cloning and overexpression of ribosomal protein L39 gene from deltamethrin-resistant Culex pipiens pallens",

abstract = "The complete sequence of ribosomal protein L39 has been cloned from deltamethrin-resistant strain of Culex pipiens pallens (DR1 strain). Quantitative RT-PCR analysis indicated that the RPL39 transcription level was 23.4 times higher in DR1 strain than in susceptible strain at 4th instar larvae. The RPL39 expression was also found to be consistently higher throughout the life cycle of DR1 strain. A protein of predicted size 17 kDa has been detected by Western blotting in RPL39-transfected mosquito C6/36 cells. These RPL39-transfected cells also showed enhanced deltamethrin resistance compared to plasmid vector-transfected cells as determined by methyl tritiated thymidine (3H-TdR) incorporation. These results indicate that RPL39 is expressed at higher levels in DR1 strain, and may confer some insecticide resistance in Cx. pipiens pallens.",

author = "Wenbin Tan and Lixin Sun and Donghui Zhang and Jing Sun and Jin Qian and Xiaobang Hu and Weijie Wang and Yan Sun and Lei Ma and Changliang Zhu",

note = "Funding Information: The work was part supported by grants from the Chinese Natural Science Research Council (No. 30371255 to Changliang Zhu), Chinese College doctorial foundation (No. 2005312004) and College and University Natural Science Foundation of Jiangsu Province (No. BK2004147 to Changliang Zhu).",

year = "2007",

month = apr,

doi = "10.1016/j.exppara.2006.09.020",

language = "English (US)",

volume = "115",

pages = "369--378",

journal = "Experimental Parasitology",

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T1 - Cloning and overexpression of ribosomal protein L39 gene from deltamethrin-resistant Culex pipiens pallens

AU - Tan, Wenbin

AU - Sun, Lixin

AU - Zhang, Donghui

AU - Sun, Jing

AU - Qian, Jin

AU - Hu, Xiaobang

AU - Wang, Weijie

AU - Sun, Yan

AU - Ma, Lei

AU - Zhu, Changliang

N1 - Funding Information: The work was part supported by grants from the Chinese Natural Science Research Council (No. 30371255 to Changliang Zhu), Chinese College doctorial foundation (No. 2005312004) and College and University Natural Science Foundation of Jiangsu Province (No. BK2004147 to Changliang Zhu).

PY - 2007/4

Y1 - 2007/4

N2 - The complete sequence of ribosomal protein L39 has been cloned from deltamethrin-resistant strain of Culex pipiens pallens (DR1 strain). Quantitative RT-PCR analysis indicated that the RPL39 transcription level was 23.4 times higher in DR1 strain than in susceptible strain at 4th instar larvae. The RPL39 expression was also found to be consistently higher throughout the life cycle of DR1 strain. A protein of predicted size 17 kDa has been detected by Western blotting in RPL39-transfected mosquito C6/36 cells. These RPL39-transfected cells also showed enhanced deltamethrin resistance compared to plasmid vector-transfected cells as determined by methyl tritiated thymidine (3H-TdR) incorporation. These results indicate that RPL39 is expressed at higher levels in DR1 strain, and may confer some insecticide resistance in Cx. pipiens pallens.

AB - The complete sequence of ribosomal protein L39 has been cloned from deltamethrin-resistant strain of Culex pipiens pallens (DR1 strain). Quantitative RT-PCR analysis indicated that the RPL39 transcription level was 23.4 times higher in DR1 strain than in susceptible strain at 4th instar larvae. The RPL39 expression was also found to be consistently higher throughout the life cycle of DR1 strain. A protein of predicted size 17 kDa has been detected by Western blotting in RPL39-transfected mosquito C6/36 cells. These RPL39-transfected cells also showed enhanced deltamethrin resistance compared to plasmid vector-transfected cells as determined by methyl tritiated thymidine (3H-TdR) incorporation. These results indicate that RPL39 is expressed at higher levels in DR1 strain, and may confer some insecticide resistance in Cx. pipiens pallens.

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JO - Experimental Parasitology

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Cloning and overexpression of ribosomal protein L39 gene from deltamethrin-resistant Culex pipiens pallens

Abstract

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