The Escherichia coli gene encoding a regulator of stringent response and virulence, DksA, which contains a canonical Zn finger motif, was cloned and expressed, and the purified protein was crystallized by the hanging-drop vapox-diffusion technique in two different space groups, P212 121 (a = 91.32, b = 96.59, c = 117.48 Å) and C222 (a = 80.6, b = 115.1, c = 149.57 Å). The crystals belonging to space group P212121, improved by macroseeding, diffract beyond 2.0 Å at a synchrotron. Three complete atomic resolution multiple anomalous dispersion diffraction data sets were collected from the same crystal of the P212121 crystal form at the absorption edge for Zn atoms.
|Original language||English (US)|
|Number of pages||3|
|Journal||Acta Crystallographica Section D: Biological Crystallography|
|State||Published - Sep 2004|
All Science Journal Classification (ASJC) codes
- Structural Biology