TY - JOUR
T1 - Cloning, mapping, and developmental expression of a sixth zebrafish Na,K-ATPase α1 subunit gene (atp1a1a.5)
AU - Blasiole, Brian
AU - Canfield, Victor
AU - Degrave, Agnes
AU - Thisse, Christine
AU - Thisse, Bernard
AU - Rajarao, Johannes
AU - Levenson, Robert
N1 - Funding Information:
This work was supported by NIH grants to B.T. and R.L. B.T. and C.T. acknowledge support from the Institut National de la Santé et de la Recherche Médicale, the Centre National de la Recherche Scientifique, the Hôpital Universitaire de Strasbourg, the Association pour la Recherche sur le Cancer and the Ligue Nationale Contre le Cancer. We are grateful to Drs. Keith Cheng and Manzoor Mohideen for continued help, support, and advice.
PY - 2002/12
Y1 - 2002/12
N2 - We have identified and characterized cDNAs encoding a novel zebrafish Na,K-ATPase α subunit. The full-length cDNA encodes a 1023-amino-acid-long peptide which shows greatest homology to zebrafish α1 polypeptides. Radiation hybrid mapping localized the new gene (atp1a1a.5) to linkage group 1 in close proximity to the previously identified cluster of Na,K-ATPase α1 genes. The expression of atp1a1a.5 in zebrafish embryos was analyzed using whole-mount in situ hybridization. From mid-somitogenesis through 48 h post fertilization (hpf), atp1a1a.5 transcripts were detected in the pronephric duct, ear, and mucous cells. This expression pattern continues through 108 hpf, when high levels of expression were also detected in the intestinal bulb.
AB - We have identified and characterized cDNAs encoding a novel zebrafish Na,K-ATPase α subunit. The full-length cDNA encodes a 1023-amino-acid-long peptide which shows greatest homology to zebrafish α1 polypeptides. Radiation hybrid mapping localized the new gene (atp1a1a.5) to linkage group 1 in close proximity to the previously identified cluster of Na,K-ATPase α1 genes. The expression of atp1a1a.5 in zebrafish embryos was analyzed using whole-mount in situ hybridization. From mid-somitogenesis through 48 h post fertilization (hpf), atp1a1a.5 transcripts were detected in the pronephric duct, ear, and mucous cells. This expression pattern continues through 108 hpf, when high levels of expression were also detected in the intestinal bulb.
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U2 - 10.1016/S0925-4773(03)00118-7
DO - 10.1016/S0925-4773(03)00118-7
M3 - Article
C2 - 14516687
AN - SCOPUS:4644308410
SN - 0925-4773
VL - 119
SP - S211-S214
JO - Mechanisms of Development
JF - Mechanisms of Development
IS - SUPPL. 1
ER -