TY - JOUR
T1 - Cloning, sequencing, chromosomal location, and function of cDNAs encoding an opioid growth factor receptor (OGFr) in humans
AU - Zagon, Ian S.
AU - Verderame, Michael F.
AU - Allen, Sandra S.
AU - McLaughlin, Patricia J.
N1 - Funding Information:
This work was supported by NIH grant CA66783 and the Laverty Foundation. We thank Dr. Roger J. Levin, Division of Otolaryngology, and Dr. Mark J. Jarowenko, Division of Urology, Department of Surgery for tissue specimens, and Jennifer Lehman for technical assistance.
PY - 2000
Y1 - 2000
N2 - The native opioid growth factor (OGF), [Met5]-enkephalin, is a tonic inhibitory peptide that modulates cell proliferation and tissue organization during development, cancer, cellular renewal, wound healing, and angiogenesis. OGF action is mediated by a receptor mechanism. We have cloned and sequenced cDNAs encoding multiple spliced forms of a human OGF receptor. The open reading frame in the longest cDNA was found to encode a protein of 697 amino acids, and 8 imperfect repeats of 20 amino acids each were a prominent feature. Altogether, five alternatively spliced forms were observed. The cDNA hybridized to mRNA from a variety of normal and neoplastic cells and tissues. Functional studies using antisense oligonucleotides to OGFr demonstrated an enhancement in cell growth. Fluorescent in situ hybridization (FISH) experiments showed the chromosomal location to be 20q13.3. This OGF receptor has no homology to classical opioid receptors. These results provide molecular validity for the interaction of OGF and OGF receptor in the regulation of growth processes in humans. Copyright (C) 2000 Elsevier Science B.V.
AB - The native opioid growth factor (OGF), [Met5]-enkephalin, is a tonic inhibitory peptide that modulates cell proliferation and tissue organization during development, cancer, cellular renewal, wound healing, and angiogenesis. OGF action is mediated by a receptor mechanism. We have cloned and sequenced cDNAs encoding multiple spliced forms of a human OGF receptor. The open reading frame in the longest cDNA was found to encode a protein of 697 amino acids, and 8 imperfect repeats of 20 amino acids each were a prominent feature. Altogether, five alternatively spliced forms were observed. The cDNA hybridized to mRNA from a variety of normal and neoplastic cells and tissues. Functional studies using antisense oligonucleotides to OGFr demonstrated an enhancement in cell growth. Fluorescent in situ hybridization (FISH) experiments showed the chromosomal location to be 20q13.3. This OGF receptor has no homology to classical opioid receptors. These results provide molecular validity for the interaction of OGF and OGF receptor in the regulation of growth processes in humans. Copyright (C) 2000 Elsevier Science B.V.
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U2 - 10.1016/S0006-8993(99)02330-6
DO - 10.1016/S0006-8993(99)02330-6
M3 - Article
C2 - 10677613
AN - SCOPUS:0033961207
SN - 0006-8993
VL - 856
SP - 75
EP - 83
JO - Brain research
JF - Brain research
IS - 1-2
ER -