TY - JOUR
T1 - Cloud-point extraction and capillary electrochromatography
T2 - An approach for the analysis of selected environmental toxicants in spiked human serum
AU - Sirimanne, Sarath R.
AU - Barr, John R.
AU - Patterson, Donald G.
PY - 1999/1/1
Y1 - 1999/1/1
N2 - The use of cloud-point extraction (CPE) coupled with capillary electrochromatography (CEC) as an approach for the analysis of environmental toxicants in human serum was explored. Human serum samples spiked with polycyclic aromatic hydrocarbons, polychlorinated dibenzo-p-dioxins, or phthalates were extracted using Genapol X-080 and 25% NaCl at 46°C for 5 min. The surfactant-rich phase was treated with acetonitrile to remove some of the interfering coextractants and then analyzed by CEC. The analytes within these three groups were well separated via this technique even though the presence of the residual coextractants/surfactant shifted the migration time slightly. The use of CPE-CEC proved to be a viable approach for environmental toxicant analysis. In addition, both CPE and CEC require relatively small amounts of organic solvents compared with conventional sample preparation techniques, and thus are more environmentally friendly.
AB - The use of cloud-point extraction (CPE) coupled with capillary electrochromatography (CEC) as an approach for the analysis of environmental toxicants in human serum was explored. Human serum samples spiked with polycyclic aromatic hydrocarbons, polychlorinated dibenzo-p-dioxins, or phthalates were extracted using Genapol X-080 and 25% NaCl at 46°C for 5 min. The surfactant-rich phase was treated with acetonitrile to remove some of the interfering coextractants and then analyzed by CEC. The analytes within these three groups were well separated via this technique even though the presence of the residual coextractants/surfactant shifted the migration time slightly. The use of CPE-CEC proved to be a viable approach for environmental toxicant analysis. In addition, both CPE and CEC require relatively small amounts of organic solvents compared with conventional sample preparation techniques, and thus are more environmentally friendly.
UR - http://www.scopus.com/inward/record.url?scp=0000662875&partnerID=8YFLogxK
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M3 - Article
AN - SCOPUS:0000662875
SN - 1040-7685
VL - 11
SP - 109
EP - 116
JO - Journal of Microcolumn Separations
JF - Journal of Microcolumn Separations
IS - 2
ER -