TY - JOUR
T1 - Cocaine- and amphetamine-regulated transcript peptide immunoreactivity in the brain of the CCK-1 receptor deficient obese OLETF rat
AU - Abraham, Hajnalka
AU - Covasa, Mihai
AU - Hajnal, Andras
N1 - Funding Information:
Acknowledgments The authors wish to thank Otsuka Pharmaceutical Co. (Tokushima, Japan) for the generous donation of the OLETF of the amygdala; rost-BLA rostral aspect of the basolateral nucleus/ complex of the amygdala; rost-mNTS rostral portion of the medial part of the nucleus tractus solitarii; caud-mNTS caudal part of the medial part of the nucleus tractus solitarii, lPBN lateral subdivision of the par-abrachial nucleus, elPBN external lateral subdivision of the parabra-chial nucleus, mPBN medial parabrachial nucleus. #p < 0.05; *p < 0.02, **p < 0.01,***p < 0.001. Error bars indicate mean standard error and LETO animals used to perform this study. The authors also thank Mr. N. K. Acharya and Mrs. Nelli Horvath for their excellent assistance with the oral glucose tolerance tests and histology, respectively. This research was supported by National Institute of Diabetes & Digestive & Kidney Diseases Grant DK065709 (Dr. Hajnal) and by grant of the University of Pécs PTE ÁOK-KA/2009 (Dr. Abraham). Dr. Abraham was a recipient of the Hungarian State Eötvös Scholarship.
PY - 2009/7
Y1 - 2009/7
N2 - Cocaine- and amphetamine-regulated transcript (CART) peptide is expressed in brain areas involved in homeostatic regulation and reward. CART has been shown to reduce food intake, but the underlying mechanisms and the relevance of this effect on obesity yet remain unknown. Therefore, we used immunohistochemistry to investigate the expression of CART peptide in various brain regions of the obese Otsuka Long Evans Tokushima Fatty (OLETF) rats lacking the CCK-1 receptor. Analysis revealed that whereas the distribution of CART-peptide immunoreactive neurons and axonal networks was identical in OLETF rats and lean controls, the intensity of CART immunoreactivity was significantly reduced in the rostral part of the nucleus accumbens (p < 0.01), the basolateral complex of the amygdala (p < 0.05) and the rostro-medial nucleus of the solitary tract (p < 0.001) of the OLETF rats. These areas are involved in reward and integration of taste and viscerosensory information and have been previously associated with altered functions in this strain. The findings suggest that in addition to previously described deficits in peripheral satiety signals and augmented orexigenic regulation, the anorectic effect of CART peptide may also be diminished in OLETF rats.
AB - Cocaine- and amphetamine-regulated transcript (CART) peptide is expressed in brain areas involved in homeostatic regulation and reward. CART has been shown to reduce food intake, but the underlying mechanisms and the relevance of this effect on obesity yet remain unknown. Therefore, we used immunohistochemistry to investigate the expression of CART peptide in various brain regions of the obese Otsuka Long Evans Tokushima Fatty (OLETF) rats lacking the CCK-1 receptor. Analysis revealed that whereas the distribution of CART-peptide immunoreactive neurons and axonal networks was identical in OLETF rats and lean controls, the intensity of CART immunoreactivity was significantly reduced in the rostral part of the nucleus accumbens (p < 0.01), the basolateral complex of the amygdala (p < 0.05) and the rostro-medial nucleus of the solitary tract (p < 0.001) of the OLETF rats. These areas are involved in reward and integration of taste and viscerosensory information and have been previously associated with altered functions in this strain. The findings suggest that in addition to previously described deficits in peripheral satiety signals and augmented orexigenic regulation, the anorectic effect of CART peptide may also be diminished in OLETF rats.
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U2 - 10.1007/s00221-009-1885-3
DO - 10.1007/s00221-009-1885-3
M3 - Article
C2 - 19533109
AN - SCOPUS:67650747306
SN - 0014-4819
VL - 196
SP - 545
EP - 556
JO - Experimental Brain Research
JF - Experimental Brain Research
IS - 4
ER -