TY - JOUR
T1 - Collaborative non-self recognition system in S-RNase-based self-incompatibility
AU - Kubo, Ken Ichi
AU - Entani, Tetsuyuki
AU - Takara, Akie
AU - Wang, Ning
AU - Fields, Allison M.
AU - Hua, Zhihua
AU - Toyoda, Mamiko
AU - Kawashima, Shin Ichi
AU - Ando, Toshio
AU - Isogai, Akira
AU - Kao, Teh Hui
AU - Takayama, Seiji
PY - 2010/11/5
Y1 - 2010/11/5
N2 - Self-incompatibility in flowering plants prevents inbreeding and promotes outcrossing to generate genetic diversity. In Solanaceae, a multiallelic gene, S-locus F-box (SLF), was previously shown to encode the pollen determinant in self-incompatibility. It was postulated that an SLF allelic product specifically detoxifies its non-self S-ribonucleases (S-RNases), allelic products of the pistil determinant, inside pollen tubes via the ubiquitin - 26S-proteasome system, thereby allowing compatible pollinations. However, it remained puzzling how SLF, with much lower allelic sequence diversity than S-RNase, might have the capacity to recognize a large repertoire of non-self S-RNases. We used in vivo functional assays and protein interaction assays to show that in Petunia, at least three types of divergent SLF proteins function as the pollen determinant, each recognizing a subset of non-self S-RNases. Our findings reveal a collaborative non-self recognition system in plants.
AB - Self-incompatibility in flowering plants prevents inbreeding and promotes outcrossing to generate genetic diversity. In Solanaceae, a multiallelic gene, S-locus F-box (SLF), was previously shown to encode the pollen determinant in self-incompatibility. It was postulated that an SLF allelic product specifically detoxifies its non-self S-ribonucleases (S-RNases), allelic products of the pistil determinant, inside pollen tubes via the ubiquitin - 26S-proteasome system, thereby allowing compatible pollinations. However, it remained puzzling how SLF, with much lower allelic sequence diversity than S-RNase, might have the capacity to recognize a large repertoire of non-self S-RNases. We used in vivo functional assays and protein interaction assays to show that in Petunia, at least three types of divergent SLF proteins function as the pollen determinant, each recognizing a subset of non-self S-RNases. Our findings reveal a collaborative non-self recognition system in plants.
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U2 - 10.1126/science.1195243
DO - 10.1126/science.1195243
M3 - Article
C2 - 21051632
AN - SCOPUS:78149356288
SN - 0036-8075
VL - 330
SP - 796
EP - 799
JO - Science
JF - Science
IS - 6005
ER -