TY - JOUR
T1 - Combined effect of diethyldithiocarbamate (DDC) and modest hyperthermia on chinese hamster (V79) cell survival and DNA strand break repair following photon irradiation
AU - Wagner, Henry
AU - Parkinson, David R.
AU - Madoc-Jones, Hywel
AU - Sternick, Edward S.
AU - Vrusho, Kimberly
AU - Krasin, Frank
N1 - Funding Information:
Work done in the Department of Therapeutic Radiology was supported by PHS grant numbers 2 T32 CA09045 and CA26897 awarded by the National Cancer Institute, DHHS.
Funding Information:
??Dept. of Therapeutic Radiology. t Dept. of Medicine, Division of Hematology-Oncology. Presented at the Chemical Modifiers of Cancer Treatment Banff Springs Hotel, Banff, Canada, November 30, 1983. Work in the laboratory of D.R.P. has been supported by the Cancer Research Institute, Inc., and the Laub Memorial Fund.
PY - 1984/9
Y1 - 1984/9
N2 - Previous studies from our institution have shown that 10-4 M DDC enhances the effects of radiation and of hyperthermia treatment at 43°C on the killing of Chinese hamster (DON) cells. We herein report studies on the combined effect seen at more modest temperatures (41 °C) which can be achieved in humans by whole body heating without the need for general anesthesia. Treatment of V79 cells with DDC for 60 minutes at 37°C or 41 °C had minimal toxicity at concentrations up to 5 × 10-5 M. When cells were irradiated with single doses up to 1000 rad (137Cs, 350 rad/min), pre-incubation with 10-4 M DDC had no effect on cell survival at 37°C, but markedly decreased survival at 41 °C (D37 = 475 rad without DDC, 270 rad with DDC). The mechanism of this increased cell killing is not known. We observed, however, that there is no repair of DNA single strand breaks in DNA from irradiated V79 cells previously held at 41 °C in the presence of 10-4 M DDC. Without DDC, repair of SSB was similar at 37°C and 41°C.
AB - Previous studies from our institution have shown that 10-4 M DDC enhances the effects of radiation and of hyperthermia treatment at 43°C on the killing of Chinese hamster (DON) cells. We herein report studies on the combined effect seen at more modest temperatures (41 °C) which can be achieved in humans by whole body heating without the need for general anesthesia. Treatment of V79 cells with DDC for 60 minutes at 37°C or 41 °C had minimal toxicity at concentrations up to 5 × 10-5 M. When cells were irradiated with single doses up to 1000 rad (137Cs, 350 rad/min), pre-incubation with 10-4 M DDC had no effect on cell survival at 37°C, but markedly decreased survival at 41 °C (D37 = 475 rad without DDC, 270 rad with DDC). The mechanism of this increased cell killing is not known. We observed, however, that there is no repair of DNA single strand breaks in DNA from irradiated V79 cells previously held at 41 °C in the presence of 10-4 M DDC. Without DDC, repair of SSB was similar at 37°C and 41°C.
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U2 - 10.1016/0360-3016(84)90506-6
DO - 10.1016/0360-3016(84)90506-6
M3 - Article
C2 - 6090365
AN - SCOPUS:0021142960
SN - 0360-3016
VL - 10
SP - 1575
EP - 1579
JO - International Journal of Radiation Oncology, Biology, Physics
JF - International Journal of Radiation Oncology, Biology, Physics
IS - 9
ER -
