Commercially Available Rapid Methods for Detection of Selected Food-borne Pathogens

Wladir B. Valderrama, Edward G. Dudley, Stephanie Doores, Catherine N. Cutter

Research output: Contribution to journalReview articlepeer-review

92 Scopus citations


Generally, the enumeration and isolation of food-borne pathogens is performed using culture-dependent methods. These methods are sensitive, inexpensive, and provide both qualitative and quantitative assessment of the microorganisms present in a sample, but these are time-consuming. For this reason, researchers are developing new techniques that allow detection of food pathogens in shorter period of time. This review identifies commercially available methods for rapid detection and quantification of Listeria monocytogenes, Salmonella spp., Staphylococcus aureus, and Shiga toxin-producing Escherichia coli in food samples. Three categories are discussed: immunologically based methods, nucleic acid-based assays, and biosensors. This review describes the basic mechanism and capabilities of each method, discusses the difficulties of choosing the most convenient method, and provides an overview of the future challenges for the technology for rapid detection of microorganisms.

Original languageEnglish (US)
Pages (from-to)1519-1531
Number of pages13
JournalCritical reviews in food science and nutrition
Issue number9
StatePublished - Jul 3 2016

All Science Journal Classification (ASJC) codes

  • Food Science
  • Industrial and Manufacturing Engineering


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