Comparative ability of chicken and mammalian LHRH to release LH from rooster pituitary cells in vitro

Alan Leslie Johnson; R. W. Dickerman; J. P. Advis

doi:10.1016/0024-3205(84)90678-7

Comparative ability of chicken and mammalian LHRH to release LH from rooster pituitary cells in vitro

Alan Leslie Johnson, R. W. Dickerman, J. P. Advis

Animal Science

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6 Scopus citations

Abstract

Biological properties of homogeneous solutions of chicken (c) and mammalian (m) LHRH were compared by their ability to release LH, in vitro, from a rooster pituitary cell incubation system. Homogeneity of the two LHRH species was confirmed by High Performance Liquid Chromatography (HPLC) using linear gradients of acetonitrile and phosphate buffer. A clear HPLC separation of [Gln⁸]-LHRH (cLHRH) and [Arg⁸]-LHRH (mLHRH) was obtained, with the former having a consistantly longer retention time than the latter. cLHRH cause a greater (p<.025) in vitro release of LH at low doses (<1 ng/2×10⁵ live pituitary cells), but not at high doses (>10 ng/2×10⁵ live pituitary cells), than that caused by mLHRH. Our results indicate that rooster pituitary cells are significantly more sensitive to low doses of cLHRH than to similar doses of mLHRH, when assesed by their ability to release LH in vitro.

Original language	English (US)
Pages (from-to)	1847-1851
Number of pages	5
Journal	Life Sciences
Volume	34
Issue number	19
DOIs	https://doi.org/10.1016/0024-3205(84)90678-7
State	Published - May 7 1984

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(84)90678-7

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title = "Comparative ability of chicken and mammalian LHRH to release LH from rooster pituitary cells in vitro",

abstract = "Biological properties of homogeneous solutions of chicken (c) and mammalian (m) LHRH were compared by their ability to release LH, in vitro, from a rooster pituitary cell incubation system. Homogeneity of the two LHRH species was confirmed by High Performance Liquid Chromatography (HPLC) using linear gradients of acetonitrile and phosphate buffer. A clear HPLC separation of [Gln8]-LHRH (cLHRH) and [Arg8]-LHRH (mLHRH) was obtained, with the former having a consistantly longer retention time than the latter. cLHRH cause a greater (p<.025) in vitro release of LH at low doses (<1 ng/2×105 live pituitary cells), but not at high doses (>10 ng/2×105 live pituitary cells), than that caused by mLHRH. Our results indicate that rooster pituitary cells are significantly more sensitive to low doses of cLHRH than to similar doses of mLHRH, when assesed by their ability to release LH in vitro.",

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T1 - Comparative ability of chicken and mammalian LHRH to release LH from rooster pituitary cells in vitro

AU - Johnson, Alan Leslie

AU - Dickerman, R. W.

AU - Advis, J. P.

PY - 1984/5/7

Y1 - 1984/5/7

N2 - Biological properties of homogeneous solutions of chicken (c) and mammalian (m) LHRH were compared by their ability to release LH, in vitro, from a rooster pituitary cell incubation system. Homogeneity of the two LHRH species was confirmed by High Performance Liquid Chromatography (HPLC) using linear gradients of acetonitrile and phosphate buffer. A clear HPLC separation of [Gln8]-LHRH (cLHRH) and [Arg8]-LHRH (mLHRH) was obtained, with the former having a consistantly longer retention time than the latter. cLHRH cause a greater (p<.025) in vitro release of LH at low doses (<1 ng/2×105 live pituitary cells), but not at high doses (>10 ng/2×105 live pituitary cells), than that caused by mLHRH. Our results indicate that rooster pituitary cells are significantly more sensitive to low doses of cLHRH than to similar doses of mLHRH, when assesed by their ability to release LH in vitro.

AB - Biological properties of homogeneous solutions of chicken (c) and mammalian (m) LHRH were compared by their ability to release LH, in vitro, from a rooster pituitary cell incubation system. Homogeneity of the two LHRH species was confirmed by High Performance Liquid Chromatography (HPLC) using linear gradients of acetonitrile and phosphate buffer. A clear HPLC separation of [Gln8]-LHRH (cLHRH) and [Arg8]-LHRH (mLHRH) was obtained, with the former having a consistantly longer retention time than the latter. cLHRH cause a greater (p<.025) in vitro release of LH at low doses (<1 ng/2×105 live pituitary cells), but not at high doses (>10 ng/2×105 live pituitary cells), than that caused by mLHRH. Our results indicate that rooster pituitary cells are significantly more sensitive to low doses of cLHRH than to similar doses of mLHRH, when assesed by their ability to release LH in vitro.

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ER -

Comparative ability of chicken and mammalian LHRH to release LH from rooster pituitary cells in vitro

Abstract

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