Comparative evaluation of AmpliVue HSV 1+2 assay with ELVIS culture for detecting herpes simplex Virus 1 (HSV-1) and HSV-2 in clinical specimens

Paul A. Granato; Brenda R. Alkins; Belinda Yen-Lieberman; Wallace H. Greene; Jessica Connolly; Blake W. Buchan; Nathan A. Ledeboer

doi:10.1128/JCM.01905-15

Comparative evaluation of AmpliVue HSV 1+2 assay with ELVIS culture for detecting herpes simplex Virus 1 (HSV-1) and HSV-2 in clinical specimens

Paul A. Granato
, Brenda R. Alkins
, Belinda Yen-Lieberman
, Wallace H. Greene
, Jessica Connolly
, Blake W. Buchan
, Nathan A. Ledeboer

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The AmpliVue HSV 1+2 assay was compared to the ELVIS HSV ID and D3 Typing Culture System for the qualitative detection and differentiation of herpes simplex virus 1 (HSV-1) and HSV-2 DNA in 1,351 cutaneous and mucocutaneous specimens. Compared to ELVIS, AmpliVue had sensitivities of 95.7 and 97.6% for detecting HSV-1 and HSV-2, respectively. Following arbitration of discordant results by an independent molecular method, the AmpliVue assay had a resolved sensitivity and specificity of 99.2 and 99.7%, respectively, for both HSV-1 and HSV-2, whereas ELVIS had a resolved sensitivity of 87.1% for HSV-1 and 84.5% for HSV-2.

Original language	English (US)
Pages (from-to)	3922-3925
Number of pages	4
Journal	Journal of clinical microbiology
Volume	53
Issue number	12
DOIs	https://doi.org/10.1128/JCM.01905-15
State	Published - Dec 2015

All Science Journal Classification (ASJC) codes

Microbiology (medical)

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10.1128/JCM.01905-15

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title = "Comparative evaluation of AmpliVue HSV 1+2 assay with ELVIS culture for detecting herpes simplex Virus 1 (HSV-1) and HSV-2 in clinical specimens",

abstract = "The AmpliVue HSV 1+2 assay was compared to the ELVIS HSV ID and D3 Typing Culture System for the qualitative detection and differentiation of herpes simplex virus 1 (HSV-1) and HSV-2 DNA in 1,351 cutaneous and mucocutaneous specimens. Compared to ELVIS, AmpliVue had sensitivities of 95.7 and 97.6\% for detecting HSV-1 and HSV-2, respectively. Following arbitration of discordant results by an independent molecular method, the AmpliVue assay had a resolved sensitivity and specificity of 99.2 and 99.7\%, respectively, for both HSV-1 and HSV-2, whereas ELVIS had a resolved sensitivity of 87.1\% for HSV-1 and 84.5\% for HSV-2.",

author = "Granato, \{Paul A.\} and Alkins, \{Brenda R.\} and Belinda Yen-Lieberman and Greene, \{Wallace H.\} and Jessica Connolly and Buchan, \{Blake W.\} and Ledeboer, \{Nathan A.\}",

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AU - Granato, Paul A.

AU - Alkins, Brenda R.

AU - Yen-Lieberman, Belinda

AU - Greene, Wallace H.

AU - Connolly, Jessica

AU - Buchan, Blake W.

AU - Ledeboer, Nathan A.

PY - 2015/12

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AB - The AmpliVue HSV 1+2 assay was compared to the ELVIS HSV ID and D3 Typing Culture System for the qualitative detection and differentiation of herpes simplex virus 1 (HSV-1) and HSV-2 DNA in 1,351 cutaneous and mucocutaneous specimens. Compared to ELVIS, AmpliVue had sensitivities of 95.7 and 97.6% for detecting HSV-1 and HSV-2, respectively. Following arbitration of discordant results by an independent molecular method, the AmpliVue assay had a resolved sensitivity and specificity of 99.2 and 99.7%, respectively, for both HSV-1 and HSV-2, whereas ELVIS had a resolved sensitivity of 87.1% for HSV-1 and 84.5% for HSV-2.

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Comparative evaluation of AmpliVue HSV 1+2 assay with ELVIS culture for detecting herpes simplex Virus 1 (HSV-1) and HSV-2 in clinical specimens

Abstract

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