Comparison of competitive protein binding analysis and radioimmunoassay for the determination of cortisol in serum and urine

Laurence M. Demers; Dennis D. Derck

doi:10.1016/S0009-9120(77)91392-3

Comparison of competitive protein binding analysis and radioimmunoassay for the determination of cortisol in serum and urine

Laurence M. Demers, Dennis D. Derck

Department of Pathology and Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The determination of serum and urinary free cortisol by competitive protein binding and radioimmunoassay were compared on patient samples using two commercially available kits. In addition, the Beckman RIA kit reagents were examined for precision and accuracy through the performance of reproducibility, recovery, cross reactivity and parallelism studies on serum and urine. Analytical recovery experiments with cortisol added to urine and serum showed 90% and 97% recovery respectively with the RIA reagents. Good reproducibility and parallelism criteria were also obtained using Beckman kit reagents. Antiserum specificity was documented and demonstrated no cross reactivity with structurally related steroids at clinically normal serum concentrations.

Original language	English (US)
Pages (from-to)	104-108
Number of pages	5
Journal	Clinical Biochemistry
Volume	10
Issue number	C
DOIs	https://doi.org/10.1016/S0009-9120(77)91392-3
State	Published - 1977

All Science Journal Classification (ASJC) codes

Clinical Biochemistry

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10.1016/S0009-9120(77)91392-3

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abstract = "The determination of serum and urinary free cortisol by competitive protein binding and radioimmunoassay were compared on patient samples using two commercially available kits. In addition, the Beckman RIA kit reagents were examined for precision and accuracy through the performance of reproducibility, recovery, cross reactivity and parallelism studies on serum and urine. Analytical recovery experiments with cortisol added to urine and serum showed 90% and 97% recovery respectively with the RIA reagents. Good reproducibility and parallelism criteria were also obtained using Beckman kit reagents. Antiserum specificity was documented and demonstrated no cross reactivity with structurally related steroids at clinically normal serum concentrations.",

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AB - The determination of serum and urinary free cortisol by competitive protein binding and radioimmunoassay were compared on patient samples using two commercially available kits. In addition, the Beckman RIA kit reagents were examined for precision and accuracy through the performance of reproducibility, recovery, cross reactivity and parallelism studies on serum and urine. Analytical recovery experiments with cortisol added to urine and serum showed 90% and 97% recovery respectively with the RIA reagents. Good reproducibility and parallelism criteria were also obtained using Beckman kit reagents. Antiserum specificity was documented and demonstrated no cross reactivity with structurally related steroids at clinically normal serum concentrations.

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Comparison of competitive protein binding analysis and radioimmunoassay for the determination of cortisol in serum and urine

Abstract

All Science Journal Classification (ASJC) codes

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