TY - JOUR
T1 - Comparison of modulation of Kv1.3 channel by two receptor tyrosine kinases in olfactory bulb neurons of rodents
AU - Colley, B.
AU - Tucker, K.
AU - Fadool, Debra Ann
N1 - Funding Information:
This work was supported by a grant from the National Institutes of Health NIH R01 DC03387 from the NIDCD. We thank Mr. Chad Thorson for technical assistance in the project.
PY - 2004
Y1 - 2004
N2 - Activation of the receptor tyrosine kinase (RTK), insulin (IRK) or neurotrophin B (TrkB), was characterized and compared in olfactory bulb neuron (OBN) cultures from Sprague Dawley rats and sv129 B6 mice. Current suppression attributed to modulation of the delayed rectifier, Kv1.3, a voltage-gated potassium (Kv) channel of the Shaker family, was observed following acute application of the growth factors, insulin or brain-derived neurotrophic factor (BDNF), to mitral cells of either rodent model. Using site-directed mutagenesis of putative tyrosine phosphorylation recognition motifs in the channel, we find that stimulation of Kv1.3 with these growth factors causes multiple phosphorylation, albeit via different residue combinations that are RTK specific.
AB - Activation of the receptor tyrosine kinase (RTK), insulin (IRK) or neurotrophin B (TrkB), was characterized and compared in olfactory bulb neuron (OBN) cultures from Sprague Dawley rats and sv129 B6 mice. Current suppression attributed to modulation of the delayed rectifier, Kv1.3, a voltage-gated potassium (Kv) channel of the Shaker family, was observed following acute application of the growth factors, insulin or brain-derived neurotrophic factor (BDNF), to mitral cells of either rodent model. Using site-directed mutagenesis of putative tyrosine phosphorylation recognition motifs in the channel, we find that stimulation of Kv1.3 with these growth factors causes multiple phosphorylation, albeit via different residue combinations that are RTK specific.
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U2 - 10.1080/10606820490270870
DO - 10.1080/10606820490270870
M3 - Article
C2 - 14769549
AN - SCOPUS:1242289213
SN - 1060-6823
VL - 10
SP - 25
EP - 36
JO - Receptors and Channels
JF - Receptors and Channels
IS - 1
ER -