TY - JOUR
T1 - Comparison of neutralization of BPV‐1 infection of C127 cells and bovine fetal skin xenografts
AU - Ghim, Shinje
AU - Christensen, Neil D.
AU - Kreider, John W.
AU - Bennett Jenson, A.
PY - 1991/9/9
Y1 - 1991/9/9
N2 - BPV‐I induces focus formation in murine C127 cells and fibropapillomas in bovine fetal skin xenografts. In this study, we compared the specificity of neutralization of BPV‐I in both assay systems, using sera and monoclonal antibodies (MAbs) selected to define neutralizing epitopes. Sera from rabbits and cattle, inoculated with intact BPV‐1 or BPV‐2 virions, neutralize BPV‐1 infectivity in both C127 cells and xenografts. Selected human sera and murine MAbs that react with intact BPV‐1 particles, serum of a rabbit immunized with denatured BPV‐1 particles, and sera from calves vaccinated with a recombinant L1 fusion protein did not neutralize BPV‐1 infection in either system. It was concluded that neutralization of BPV‐1 infection of C127 cells and bovine fetal skin xenografts by hyperimmune sera is specific and concordant for both assay systems, and involves conformational BPV‐1 capsid epitopes.
AB - BPV‐I induces focus formation in murine C127 cells and fibropapillomas in bovine fetal skin xenografts. In this study, we compared the specificity of neutralization of BPV‐I in both assay systems, using sera and monoclonal antibodies (MAbs) selected to define neutralizing epitopes. Sera from rabbits and cattle, inoculated with intact BPV‐1 or BPV‐2 virions, neutralize BPV‐1 infectivity in both C127 cells and xenografts. Selected human sera and murine MAbs that react with intact BPV‐1 particles, serum of a rabbit immunized with denatured BPV‐1 particles, and sera from calves vaccinated with a recombinant L1 fusion protein did not neutralize BPV‐1 infection in either system. It was concluded that neutralization of BPV‐1 infection of C127 cells and bovine fetal skin xenografts by hyperimmune sera is specific and concordant for both assay systems, and involves conformational BPV‐1 capsid epitopes.
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U2 - 10.1002/ijc.2910490224
DO - 10.1002/ijc.2910490224
M3 - Article
C2 - 1715330
AN - SCOPUS:0025770024
SN - 0020-7136
VL - 49
SP - 285
EP - 289
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 2
ER -
