Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates

The previously presented consensus sequence for eukaryotic translation initiation sites by Kozak (1) was derived substantially from vertebrate mRNA sequences. Drosophila nuclear genes exhibit a significantly different translation start consensus sequence. These differences probably do not represent mechanistic differences in translation initiation inasmuch as both taxa exhibit identical preferences and restrictions at the crucial -3 position. Using more conservative criteria for the assignment of consensus the following consensus sequences were derived: vertebrate--CANCAUG and Drosophila--CANCAUG