TY - JOUR
T1 - Competitive recruitment of CBP and Rb-HDAC regulates UBF acetylation and ribosomal transcription
AU - Pelletier, Guillaume
AU - Stefanovsky, Victor Y.
AU - Faubladier, Marlène
AU - Hirschler-Laszkiewicz, Iwona
AU - Savard, Julie
AU - Rothblum, Lawrence I.
AU - Côté, Jacques
AU - Moss, Tom
N1 - Funding Information:
The authors wish to thank A. J. Bannister, D. Trouche, M. V. Govindan, J. K. Tong, and W. G. Kaelin for supplying the various expression vectors and for providing advice on their use. This work was supported in Canada by an operating grant from the Medical Research Council of Canada, an FCAR-FRSQ Santé scholarship to G. P., and an MRC-Canada Scientist award to T. M.; and in France by the Centre National de la Recherche Scientifique (CNRS) with grants from the Fondation pour la Recherche Médicale (FRM) and the Association pour la Recherche sur le Cancer (ARC). L .I. R. acknowledges support from the NIH, grant GM46991.
PY - 2000
Y1 - 2000
N2 - RNA polymerase I (Poll) transcription is activated by the HMG box architectural factor UBF, which loops approximately 140 bp of DNA into the enhancesome, necessitating major chromatin remodeling. Here we show that the acetyltransferase CBP is recruited to and acetylates UBF both in vitro and in vivo. CBP activates Poll transcription in vivo through its acetyltransferase domain and acetylation of UBF facilitates transcription derepression and activation in vitro. CBP activation and Rb suppression of ribosomal transcription by recruitment to UBF are mutually exclusive, regulating in vivo Poll transcription through an acetylation-decatylation 'flip-flop.' Thus, Poll transcription is regulated by protein acetylation, and the competitive recruitment of CBP and Rb.
AB - RNA polymerase I (Poll) transcription is activated by the HMG box architectural factor UBF, which loops approximately 140 bp of DNA into the enhancesome, necessitating major chromatin remodeling. Here we show that the acetyltransferase CBP is recruited to and acetylates UBF both in vitro and in vivo. CBP activates Poll transcription in vivo through its acetyltransferase domain and acetylation of UBF facilitates transcription derepression and activation in vitro. CBP activation and Rb suppression of ribosomal transcription by recruitment to UBF are mutually exclusive, regulating in vivo Poll transcription through an acetylation-decatylation 'flip-flop.' Thus, Poll transcription is regulated by protein acetylation, and the competitive recruitment of CBP and Rb.
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U2 - 10.1016/S1097-2765(00)00104-0
DO - 10.1016/S1097-2765(00)00104-0
M3 - Article
C2 - 11106745
AN - SCOPUS:0033636744
SN - 1097-2765
VL - 6
SP - 1059
EP - 1066
JO - Molecular cell
JF - Molecular cell
IS - 5
ER -