TY - JOUR
T1 - Comprehensive biorefinery of invasive European green crab (Carcinus maenas) into multiple high-value biomolecules
AU - Zhang, Yi
AU - Sully, Paola
AU - Spitzer, Lea
AU - Dutilleul, Pierre
AU - Simpson, Benjamin K.
N1 - Funding Information:
The levels of SFAs and MUFAs such as palmitic acid and oleic acid found in this study were comparable to those reported in blue crab (Callinectes sapidus), swimming crab (Portunus trituberculatus), and Chinese mitten crab (Eriocheir sinensis), while the level of n-3 PUFAs in the studied green crab oil was considerably higher than those reported in these crab species (Çelik et al., 2004; Chen et al., 2007; Cherif et al., 2008; He et al., 2017). DHA and EPA are n-3 LC-PUFA with well-established health benefits to support fetal development, cardiovascular function, and cognitive function; however, humans do not efficiently produce these fatty acids (Swanson et al., 2012). Thus, the recovered crab oil could be used in health supplements and enriched food products.This work was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Program.
Funding Information:
This work was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Program.
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/11/10
Y1 - 2022/11/10
N2 - European green crab (Carcinus maenas) is an invasive species globally and is considered marine waste that needs to be eliminated. In this study, the green crab was biorefined via a clean enzymes-assisted bioprocess into multiple value-added bioproducts, i.e., crab oil, minerals, carotenoprotein, and chitin. The recovered crab oil was rich in unsaturated fatty acids such as EPA and DHA as per GC-MS analysis. Citric acid, as a replacement for conventional hash acids, was found to optimally demineralize the crab powder at 1 M and 4 °C for 18 h, where the recovered crab minerals identified using ICP-MS showed a high level of calcium, in form of nutritious calcium citrate. Crab carotenoprotein recovered using trypsin exhibited antioxidant properties. It contained 139.26 μg/g of carotenoids pigment, mainly astaxanthin and astaxanthin diester, as verified by thin layer chromatography, and the protein component in the carotenoprotein was rich in essential amino acids such as leucine via UPLC-MS. The produced crab chitin from papain-assisted deproteinization showed characteristic physicochemical properties following FTIR and XRD analysis. These biomolecules biorefined from the green crab possess wide applications in food, pharmaceutical, biomaterials, etc. This new biorefinery strategy enhances the sustainability for biowaste utilization.
AB - European green crab (Carcinus maenas) is an invasive species globally and is considered marine waste that needs to be eliminated. In this study, the green crab was biorefined via a clean enzymes-assisted bioprocess into multiple value-added bioproducts, i.e., crab oil, minerals, carotenoprotein, and chitin. The recovered crab oil was rich in unsaturated fatty acids such as EPA and DHA as per GC-MS analysis. Citric acid, as a replacement for conventional hash acids, was found to optimally demineralize the crab powder at 1 M and 4 °C for 18 h, where the recovered crab minerals identified using ICP-MS showed a high level of calcium, in form of nutritious calcium citrate. Crab carotenoprotein recovered using trypsin exhibited antioxidant properties. It contained 139.26 μg/g of carotenoids pigment, mainly astaxanthin and astaxanthin diester, as verified by thin layer chromatography, and the protein component in the carotenoprotein was rich in essential amino acids such as leucine via UPLC-MS. The produced crab chitin from papain-assisted deproteinization showed characteristic physicochemical properties following FTIR and XRD analysis. These biomolecules biorefined from the green crab possess wide applications in food, pharmaceutical, biomaterials, etc. This new biorefinery strategy enhances the sustainability for biowaste utilization.
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U2 - 10.1016/j.jclepro.2022.134052
DO - 10.1016/j.jclepro.2022.134052
M3 - Article
AN - SCOPUS:85138756976
SN - 0959-6526
VL - 374
JO - Journal of Cleaner Production
JF - Journal of Cleaner Production
M1 - 134052
ER -