TY - JOUR
T1 - Conservation of NON-EXPRESSOR OF PATHOGENESIS-RELATED GENES1 function between Arabidopsis thaliana and Brassica napus
AU - Potlakayala, Shobha D.
AU - DeLong, Catherine
AU - Sharpe, Andrew
AU - Fobert, Pierre R.
N1 - Funding Information:
We thank Dr. Roger Rimmer (Agriculture and Agri-Food Canada, Saskatoon, Sask.) for help with statistical analysis, Drs. Robin Cameron (McMaster University, Hamilton, Ont.) and John Taylor (Horticulture Research International, Wellesbourne, UK) for providing P. syringae pv. tomato DC3000 and P. syringae pv. maculicola , respectively, Dr. Isobel Parkin for help searching the AAFC EST collection, and Drs. Mark Smith, Yangdou Wei and Guosheng Liu for critically reading the manuscript. This research was supported by the National Research Council Plant Biotechnology Institute core funding, the National Research Council Genomics and Health Initiative, the National Science and Engineering Research Council (NSERC) discovery grant program, a University of Saskatchewan New Faculty Dean Scholarship and Graduate Teaching Fellowship. The AAFC Canadian Crop Genomics Initiative supported the development of a B. napus EST collection at the Saskatoon Research Centre. This is National Research Council Canada publication #48444.
PY - 2007
Y1 - 2007
N2 - An Expressed Sequence Tag encoding a protein highly related to Arabidopsis NPR1 was isolated from canola quality Brassica napus. The protein, BnNPR1, interacts with the same spectrum of TGA transcription factors and NIMIN proteins as AtNPR1 in the yeast two-hybrid system. When expressed in Arabidopsis npr1 mutants, BnNPR1 restored salicylic acid-dependent expression of the marker gene PR-1 and enhanced basal defense as well as systemic acquired resistance against a virulent strain of the bacterial pathogen Pseudomonas syringae. Expression of Arabidopsis NPR1 or over expression of BnNPR1 in transgenic B. napus also effectively enhanced basal resistance against P. syringae. Crown
AB - An Expressed Sequence Tag encoding a protein highly related to Arabidopsis NPR1 was isolated from canola quality Brassica napus. The protein, BnNPR1, interacts with the same spectrum of TGA transcription factors and NIMIN proteins as AtNPR1 in the yeast two-hybrid system. When expressed in Arabidopsis npr1 mutants, BnNPR1 restored salicylic acid-dependent expression of the marker gene PR-1 and enhanced basal defense as well as systemic acquired resistance against a virulent strain of the bacterial pathogen Pseudomonas syringae. Expression of Arabidopsis NPR1 or over expression of BnNPR1 in transgenic B. napus also effectively enhanced basal resistance against P. syringae. Crown
UR - http://www.scopus.com/inward/record.url?scp=46549083728&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=46549083728&partnerID=8YFLogxK
U2 - 10.1016/j.pmpp.2008.01.003
DO - 10.1016/j.pmpp.2008.01.003
M3 - Article
AN - SCOPUS:46549083728
SN - 0885-5765
VL - 71
SP - 174
EP - 183
JO - Physiological and Molecular Plant Pathology
JF - Physiological and Molecular Plant Pathology
IS - 4-6
ER -