Conserved MHC gene orthologs genetically map to the Turkey MHC-B

Kent M. Reed; Benjamin Benoit; Xiong Wang; Molly A. Greenshields; Camilla H.K. Hughes; Kristelle M. Mendoza

doi:10.1159/000366440

Conserved MHC gene orthologs genetically map to the Turkey MHC-B

Kent M. Reed, Benjamin Benoit, Xiong Wang, Molly A. Greenshields, Camilla H.K. Hughes, Kristelle M. Mendoza

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The avian MHC-associated gene set includes orthologs to genes found throughout the human major histocompatibility complex (MHC), including some loci of the evolutionarily conserved class III region. In the turkey and other Galliformes, genes linked to the MHC have been identified because they are closely associated with class I or class II genes. This study was designed to evaluate additional class III genes for linkage to the avian MHC to further determine conservation of these loci in birds. BLAST searches were used to locate sequences in the turkey genome with similarity to genes shared between the MHC of Xenopus and humans. Primers were designed to target 25 genes, and putative orthologs were amplified by PCR and sequenced. Sequence polymorphisms were identified for 15 genes in turkey reference mapping families, and 8 genes showed significant genetic linkage to the turkey MHC-B locus.

Original language	English (US)
Pages (from-to)	31-38
Number of pages	8
Journal	Cytogenetic and Genome Research
Volume	144
Issue number	1
DOIs	https://doi.org/10.1159/000366440
State	Published - Nov 19 2014

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics
Genetics(clinical)

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title = "Conserved MHC gene orthologs genetically map to the Turkey MHC-B",

abstract = "The avian MHC-associated gene set includes orthologs to genes found throughout the human major histocompatibility complex (MHC), including some loci of the evolutionarily conserved class III region. In the turkey and other Galliformes, genes linked to the MHC have been identified because they are closely associated with class I or class II genes. This study was designed to evaluate additional class III genes for linkage to the avian MHC to further determine conservation of these loci in birds. BLAST searches were used to locate sequences in the turkey genome with similarity to genes shared between the MHC of Xenopus and humans. Primers were designed to target 25 genes, and putative orthologs were amplified by PCR and sequenced. Sequence polymorphisms were identified for 15 genes in turkey reference mapping families, and 8 genes showed significant genetic linkage to the turkey MHC-B locus.",

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AU - Reed, Kent M.

AU - Benoit, Benjamin

AU - Wang, Xiong

AU - Greenshields, Molly A.

AU - Hughes, Camilla H.K.

AU - Mendoza, Kristelle M.

PY - 2014/11/19

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AB - The avian MHC-associated gene set includes orthologs to genes found throughout the human major histocompatibility complex (MHC), including some loci of the evolutionarily conserved class III region. In the turkey and other Galliformes, genes linked to the MHC have been identified because they are closely associated with class I or class II genes. This study was designed to evaluate additional class III genes for linkage to the avian MHC to further determine conservation of these loci in birds. BLAST searches were used to locate sequences in the turkey genome with similarity to genes shared between the MHC of Xenopus and humans. Primers were designed to target 25 genes, and putative orthologs were amplified by PCR and sequenced. Sequence polymorphisms were identified for 15 genes in turkey reference mapping families, and 8 genes showed significant genetic linkage to the turkey MHC-B locus.

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Conserved MHC gene orthologs genetically map to the Turkey MHC-B

Abstract

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