Construction and characterization of a single-chain catalytic antibody

Richard A. Gibbs; Bruce A. Posner; David R. Filpula; Steven W. Dodd; Malcolm A.J. Finkelman; Timothy K. Lee; Marie Wroble; Marc Whitlow; Stephen J. Benkovic

doi:10.1073/pnas.88.9.4001

Construction and characterization of a single-chain catalytic antibody

Richard A. Gibbs, Bruce A. Posner, David R. Filpula, Steven W. Dodd, Malcolm A.J. Finkelman, Timothy K. Lee, Marie Wroble, Marc Whitlow, Stephen J. Benkovic

Chemistry

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

The antigen-binding (Fab) fragment of the catalytic monoclonal antibody NPN43C9 has recently been cloned by using bacteriophage λ. By inserting the variable regions of this Fab coding sequence into a (NH₂)-V_L-linker-V_H-(COOH) construct (where V_L and V_H represent the heavy and light chain variable regions), we have assembled a recombinant gene encoding a catalytic single-chain antigen-binding protein. This protein has been expressed in Escherichia coli and exhibits the same catalytic parameters as the parent monoclonal antibody NPN43C9. Single-chain forms of catalytic antibodies may prove valuable for structural and site-directed mutagenesis studies as well as for large-scale applications of catalytic antibodies.

Original language	English (US)
Pages (from-to)	4001-4004
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	88
Issue number	9
DOIs	https://doi.org/10.1073/pnas.88.9.4001
State	Published - 1991

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10.1073/pnas.88.9.4001

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title = "Construction and characterization of a single-chain catalytic antibody",

abstract = "The antigen-binding (Fab) fragment of the catalytic monoclonal antibody NPN43C9 has recently been cloned by using bacteriophage λ. By inserting the variable regions of this Fab coding sequence into a (NH2)-VL-linker-VH-(COOH) construct (where VL and VH represent the heavy and light chain variable regions), we have assembled a recombinant gene encoding a catalytic single-chain antigen-binding protein. This protein has been expressed in Escherichia coli and exhibits the same catalytic parameters as the parent monoclonal antibody NPN43C9. Single-chain forms of catalytic antibodies may prove valuable for structural and site-directed mutagenesis studies as well as for large-scale applications of catalytic antibodies.",

author = "Gibbs, {Richard A.} and Posner, {Bruce A.} and Filpula, {David R.} and Dodd, {Steven W.} and Finkelman, {Malcolm A.J.} and Lee, {Timothy K.} and Marie Wroble and Marc Whitlow and Benkovic, {Stephen J.}",

year = "1991",

doi = "10.1073/pnas.88.9.4001",

language = "English (US)",

volume = "88",

pages = "4001--4004",

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T1 - Construction and characterization of a single-chain catalytic antibody

AU - Gibbs, Richard A.

AU - Posner, Bruce A.

AU - Filpula, David R.

AU - Dodd, Steven W.

AU - Finkelman, Malcolm A.J.

AU - Lee, Timothy K.

AU - Wroble, Marie

AU - Whitlow, Marc

AU - Benkovic, Stephen J.

PY - 1991

Y1 - 1991

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AB - The antigen-binding (Fab) fragment of the catalytic monoclonal antibody NPN43C9 has recently been cloned by using bacteriophage λ. By inserting the variable regions of this Fab coding sequence into a (NH2)-VL-linker-VH-(COOH) construct (where VL and VH represent the heavy and light chain variable regions), we have assembled a recombinant gene encoding a catalytic single-chain antigen-binding protein. This protein has been expressed in Escherichia coli and exhibits the same catalytic parameters as the parent monoclonal antibody NPN43C9. Single-chain forms of catalytic antibodies may prove valuable for structural and site-directed mutagenesis studies as well as for large-scale applications of catalytic antibodies.

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JF - Proceedings of the National Academy of Sciences of the United States of America

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Construction and characterization of a single-chain catalytic antibody

Abstract

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