Construction of RNA nanocages by re-engineering the packaging RNA of Phi29 bacteriophage

Chenhui Hao; Xiang Li; Cheng Tian; Wen Jiang; Guansong Wang; Chengde Mao

doi:10.1038/ncomms4890

Construction of RNA nanocages by re-engineering the packaging RNA of Phi29 bacteriophage

Chenhui Hao, Xiang Li, Cheng Tian, Wen Jiang, Guansong Wang, Chengde Mao

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69 Scopus citations

Abstract

RNA nanotechnology promises rational design of RNA nanostructures with wide array of structural diversities and functionalities. Such nanostructures could be used in applications such as small interfering RNA delivery and organization of in vivo chemical reactions. Though having impressive development in recent years, RNA nanotechnology is still quite limited and its programmability and complexity could not rival the degree of its closely related cousin: DNA nanotechnology. Novel strategies are needed for programmed RNA self-assembly. Here, we have assembled RNA nanocages by re-engineering a natural, biological RNA motif: the packaging RNA of phi29 bacteriophage. The resulting RNA nanostructures have been thoroughly characterized by gel electrophoresis, cryogenic electron microscopy imaging and dynamic light scattering. copyright

Original language	English (US)
Article number	3890
Journal	Nature communications
Volume	5
DOIs	https://doi.org/10.1038/ncomms4890
State	Published - May 16 2014

All Science Journal Classification (ASJC) codes

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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abstract = "RNA nanotechnology promises rational design of RNA nanostructures with wide array of structural diversities and functionalities. Such nanostructures could be used in applications such as small interfering RNA delivery and organization of in vivo chemical reactions. Though having impressive development in recent years, RNA nanotechnology is still quite limited and its programmability and complexity could not rival the degree of its closely related cousin: DNA nanotechnology. Novel strategies are needed for programmed RNA self-assembly. Here, we have assembled RNA nanocages by re-engineering a natural, biological RNA motif: the packaging RNA of phi29 bacteriophage. The resulting RNA nanostructures have been thoroughly characterized by gel electrophoresis, cryogenic electron microscopy imaging and dynamic light scattering. copyright",

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AU - Mao, Chengde

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Construction of RNA nanocages by re-engineering the packaging RNA of Phi29 bacteriophage

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